4.1.2.4: deoxyribose-phosphate aldolase
This is an abbreviated version!
For detailed information about deoxyribose-phosphate aldolase, go to the full flat file.
Word Map on EC 4.1.2.4
Reaction
Synonyms
2-deoxy-D-ribose 5-phosphate aldolase, 2-deoxy-D-ribose-5-phosphate aldolase, 2-deoxyribose 5-phosphate aldolase, 2-Deoxyribose-5-phosphate aldolase, aldolase, deoxyribo, CGI-26, D-2-deoxyribose-5-phosphate aldolase, d5RP aldolase, DeoC, Deoxyriboaldolase, deoxyribose 5-phosphate aldolase, deoxyribose phosphate aldolase-like protein, Deoxyribose-5-phosphate aldolase, deoxyribose-phosphate aldolase, DERA, DPA, DR aldolase, Phosphodeoxyriboaldolase, TgDERA, TgDPA, Tk-DeoC, TK2104, TtDERA
ECTree
4.1.2.4 deoxyribose-phosphate aldolaseAdvanced search results
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Application on EC 4.1.2.4 - deoxyribose-phosphate aldolase
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APPLICATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
biotechnology
industry
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ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. 2-Deoxy-D-ribose-5-phosphate aldolase can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided
medicine
synthesis
biotechnology
DERA has the potential to resist high concentrations of acetaldehyde and may serve as an industrial catalyst
biotechnology
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efficient process for the production of thermophilic DERA designed from the point of view of recombinant enzyme concentration and productivity, which is economically and technically viable and can be used as a guide for production of other synthetically useful enzymes
medicine
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possible selection as a vaccine candidate. The purified recombinant protein is used to immunize rats. The antibodies obtained are used to verify in vitro expression of the enzyme. The vector pVAX1 is utilized to formulate a DNA vaccine designated as pTgDPA, which is used to evaluate the immunological changes and the level of protection against challenge with the virulent RH strain of Toxoplasma gondii. DNA vaccine, TgDPA revealed that it can induce a strong humoral as well as cellular mediated response in mice. These responses are a contribution of TH1, TH2 and TH17 type of responses. Mice immunized with TgDPA show longer survival rates than do mice in control groups
medicine
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possible selection as a vaccine candidate. The purified recombinant protein is used to immunize rats. The antibodies obtained are used to verify in vitro expression of the enzyme. The vector pVAX1 is utilized to formulate a DNA vaccine designated as pTgDPA, which is used to evaluate the immunological changes and the level of protection against challenge with the virulent RH strain of Toxoplasma gondii. DNA vaccine, TgDPA revealed that it can induce a strong humoral as well as cellular mediated response in mice. These responses are a contribution of TH1, TH2 and TH17 type of responses. Mice immunized with TgDPA show longer survival rates than do mice in control groups
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synthesis
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stereocontrolled aldol condensation of 3-hydroxy aldehydes and ketones
synthesis
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synthesis of a variety of sugar analogs, including 2-deoxy-L-fucose and analogs, thiosugars and glycolipid precursors. The sequential aldol reaction is utilized in the synthesis of a variety of 2,4-dideoxyhexoses and 2,4,6-trideoxyhexoses
synthesis
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mutant D-2-deoxyribose-5-phosphate aldolase Ser238Asp is used to prepare beta-hydroxy-delta lactol synthons and tert-butyl [(4R,6R)-6-aminoethyl-2,2-dimethyl-1,3-dioxn-4-yl]acetate, a key intermediate for atorvastatin synthesis
synthesis
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production of enzyme using a continuous lactose induction strategy. The lactose concentration in the feed medium affects directly the expression of the target protein. The use of 50 g/L in the feed medium results in an expression level of above 30%, and the maximum final enzyme concentration of 16200 U/l. The acetate concentration remains at a low level in the fed-batch phase, less than 0.5 g/l
synthesis
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biosynthesis of 2-deoxysugars using whole-cell catalyst expressing 2-deoxy-D-ribose 5-phosphate aldolase. A whole-cell transformation strategy using resting cells of the BL21(pKDERA12) strain, containing the expressed plasmid pKDERA12 (S238D/F200I/DELTAY259), results in increase in 2-deoxy-D-ribose yield from 0.41 mol/mol D-glyceraldehyde to 0.81 mol/mol D-glyceraldehyde and higher substrate tolerance from 0.5 to 3 M compared to in vitro assays. With further optimization of the transformation process, the BL21(pKDERA12) strain produces 2.14 M (287.06 g/l) 2-deoxy-D-ribose, with a yield of 0.71 mol/mol D-glyceraldehyde and average productivity of 0.13 mol/l*h (17.94 g/l*h). The results demonstrate the potential for large-scale production of 2-deoxy-D-ribose using the BL21(pKDERA12) strain. Furthermore, the BL21(pKDERA12) strain also exhibits the ability to efficiently produce 2-deoxy-D-altrose from D-erythrose, as well as 2-deoxy-L-xylose and 2-deoxy-L-ribose from L-glyceraldehyde
synthesis
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industrial application of the enzyme for the synthesis of a key building block for the pharmaceutical blockbuster atorvastatin. The main drawback of the enzyme being used in such a process is its sensitivity to industrially relevant concentrations of the substrate and the occurrence of product inhibition. Product inhibition can be avoided by coupling the catalytic transformation with transport processes, which, in turn, would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. A fabrication process for such films is developed that is based on the formation of DERA-poly(N-isopropylacrylamide) conjugates that are subsequently allowed to self-assemble at an air-water interface to yield the respective film
synthesis
synthesis of statin intermediates. The recombinant enzyme can be potentially applied in the production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose. The bioconversion process for production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant enzyme is studied and this process took 3 h for maximal conversion
synthesis
the enzyme is an interesting candidate for bio-catalysis of carbo-ligation reactions, which are central to synthetic chemistry
synthesis
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synthesis of a variety of sugar analogs, including 2-deoxy-L-fucose and analogs, thiosugars and glycolipid precursors. The sequential aldol reaction is utilized in the synthesis of a variety of 2,4-dideoxyhexoses and 2,4,6-trideoxyhexoses
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synthesis
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synthesis of statin intermediates. The recombinant enzyme can be potentially applied in the production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose. The bioconversion process for production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant enzyme is studied and this process took 3 h for maximal conversion
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