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4.1.2.38: benzoin aldolase

This is an abbreviated version!
For detailed information about benzoin aldolase, go to the full flat file.

Word Map on EC 4.1.2.38

Reaction

2-hydroxy-1,2-diphenylethanone
= 2 benzaldehyde

Synonyms

BAL, benzaldehyde lyase, benzoin aldolase, BZL, BznB, lyase, benzaldehyde

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.38 benzoin aldolase

Temperature Stability

Temperature Stability on EC 4.1.2.38 - benzoin aldolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
a concentration of 1 mM MgSO4 and 0.01 mM thiamine diphosphate is sufficient to keep BAL stable at 25°C
30 - 40
-
at 30 and 40°C in aqueous solution, the relative activity increases slightly during the first measurements, and after that the decrease is very low. At 50°C, a strong exponential decrease is observed. No significant differences of half-life between 30°C and 40°C with 16 and 18 h, respectively, whereas at 50°C, a strong decrease of the half-life is observed with 0.4 h
37
-
reaction temperature shall not exceed 37°C, in the presence of 5 mM MgSO4 and 0.1 mM thiamine diphosphate the enzyme's activity is reduced by only 15% over 3 h at 37°C, however, a concentration of 1 mM MgSO4 and 0.01 mM thiamine diphosphate leads to more than 40% inactivation within 3 h at 37°C
45.9
T50 value, i.e. 50% residual activity after 10 min
59.7
-
wild-type, T50 value, i.e. 50% residual activity after 10 min
66
-
mutant Q112L/E393Y/S416G/T479F , T50 value, i.e. 50% residual activity after 10 min
68.4
-
mutant Q112A/E393A/S416G/T479A, T50 value, i.e. 50% residual activity after 10 min