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4.1.2.38: benzoin aldolase

This is an abbreviated version!
For detailed information about benzoin aldolase, go to the full flat file.

Word Map on EC 4.1.2.38

Reaction

2-hydroxy-1,2-diphenylethanone
= 2 benzaldehyde

Synonyms

BAL, benzaldehyde lyase, benzoin aldolase, BZL, BznB, lyase, benzaldehyde

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.38 benzoin aldolase

Purification

Purification on EC 4.1.2.38 - benzoin aldolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni2+-NTA affinity column, to purity
-
immobilized metal ion chromatography, after which the enzyme shows only 35-45% of its activity, however, activity is completely regained after incubation in the presence of Mg2+ and thiamine diphosphate
-
Ni-Sepharose column chromatography
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nickel-affinity HiTrap agarose column chromatography and Sephadex S-200 gel filtration
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strain biovar I, partial
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using Ni2+-affinity chromatography
-