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4.1.2.38: benzoin aldolase

This is an abbreviated version!
For detailed information about benzoin aldolase, go to the full flat file.

Word Map on EC 4.1.2.38

Reaction

2-hydroxy-1,2-diphenylethanone
= 2 benzaldehyde

Synonyms

BAL, benzaldehyde lyase, benzoin aldolase, BZL, BznB, lyase, benzaldehyde

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.38 benzoin aldolase

General Stability

General Stability on EC 4.1.2.38 - benzoin aldolase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme entrapped in polyvinyl alcohol and suspended in hexane is stable in hexane for 1 week at 4°C and for more than 1 month at -20°C
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the half-life of BAL in the presence of the investigated solvents, but without formation of distinct solvent phases is 17.3 h, 5.6 h and 3.2 h for saturation with methyl-tert-butylether, 2-octanone, and toluene, respectively, in non-reactive monophasic media. With MTBE, 2-octanone, and toluene, very similar half-lives of 1.5 h, 1.4 h and 2.0 h are found for unmodified BAL under process conditions in reactive biphasic media. The initial activity of BAL decreases to approximately 60%, when methoxy-poly(ethylene) glycol (mPEG)750 is attached, and to 70%, when a longer-chain mPEG2000 is used. Overall, the modification with mPEG leads to good stabilization of BAL under non-reactive conditions. The strongest effect is achieved against toluene, where the stability of mPEG2000-BAL surpasses the stability of unmodified BAL by factor 18
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