4.1.2.19: rhamnulose-1-phosphate aldolase

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For detailed information about rhamnulose-1-phosphate aldolase, go to the full flat file.

Reaction

Synonyms

aldolase, rhamnulose phosphate, L-Rhamnulose-1-phosphate aldolase, L-rhamnulose-1-phosphate aldolase (class II), L-Rhamnulose-1-phosphate L-lactaldehyde-lyase, Rha-1PA, RhaD, Rhamn-1PA, rhamnulose 1-phosphate aldolase, Rhamnulose phosphate aldolase, rhamnulose-1-phosphate aldolase, RhuA, TM1072

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.19 rhamnulose-1-phosphate aldolase

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Results	in table
1	Activating Compound
3200	AA Sequence
2	Application
1	CAS Registry Number
10	Cloned(Commentary)
5	Crystallization (Commentary)
1	General Stability
1	IC50 Value
28	Inhibitors
1	kcat/KM [mM/s]
1	Ki Value [mM]
23	KM Value [mM]
36	Metals/Ions
9	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
408	Organism
7	Pathway
49	PDB ID
5	pH Optimum
4	pH Range
1	pH Stability
18	Protein Variants
11	Purification (Commentary)
1	Reaction
9	Reaction Type
33	Reference
12	Specific Activity [micromol/min/mg]
5	Storage Stability
110	Substrates and Products (Substrate)
8	Subunits
33	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 4.1.2.19 - rhamnulose-1-phosphate aldolase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

HR 16/40 afinity column chromatography

Escherichia coli

P32169

713694

Ni-IDA agarose column chromatography

Thermotoga maritima

-

746888

Ni-NTA column chromatography

Escherichia coli

-

748408

only 55% of immobilization yield is achieved when immobilized metal affinity chromatography is used to purify and immobilize RhuA from cellular lysate in a single step

Escherichia coli

-

706570

Superdex 200 gel filtration

Escherichia coli

P32169

678345

-

Escherichia coli

-

5056, 5066, 5067, 5071

-

Escherichia coli

P32169

654067, 654673