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4.1.2.14: 2-dehydro-3-deoxy-phosphogluconate aldolase

This is an abbreviated version!
For detailed information about 2-dehydro-3-deoxy-phosphogluconate aldolase, go to the full flat file.

Word Map on EC 4.1.2.14

Reaction

2-dehydro-3-deoxy-6-phospho-D-gluconate
=
pyruvate
+
D-glyceraldehyde 3-phosphate

Synonyms

2-dehydro-3-deoxy-phosphogluconate aldolase, 2-dehydro-3-deoxyphosphogluconate aldolase, 2-keto-3-deoxy-(6-phospho)-gluconate aldolase, 2-keto-3-deoxy-6-phosphogluconate aldolase, 2-keto-3-deoxy-6-phosphogluconic aldolase, 2-keto-3-deoxygluconate-6-P-aldolase, 2-keto-3-deoxygluconate-6-phosphate aldolase, 2-oxo-3-deoxy-6-phosphogluconate aldolase, 6-phospho-2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde-3-phosphate-lyase, 6-Phospho-2-keto-3-deoxygluconate aldolase, aldolase, phospho-2-keto-3-deoxygluconate, eda, KD(P)G aldolase, KDGA, KDPG aldolase, KDPG-aldolase, KDPGA, KDPGlc aldolase, ODPG aldolase, Phospho-2-dehydro-3-deoxygluconate aldolase, Phospho-2-keto-3-deoxygluconate aldolase, Phospho-2-keto-3-deoxygluconic aldolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.14 2-dehydro-3-deoxy-phosphogluconate aldolase

Purification

Purification on EC 4.1.2.14 - 2-dehydro-3-deoxy-phosphogluconate aldolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-ion affinity chromatography and ion exchange column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni2+-charged chelating Sepharose Fast Flow column chromatography
-
one-step procedure
-
purified by using Ni2+ affinity column
-
using a nickel-affinity and a Superdex 200 column