stereospecific formation of carbon-carbon bonds is one of the major interests in organic synthetic chemistry, aldolases are part of the most important group of asymmetric C-C-bonding enzymes
stereospecific formation of carbon-carbon bonds is one of the major interests in organic synthetic chemistry, aldolases are part of the most important group of asymmetric C-C-bonding enzymes
use of a recombinant strain of Ralstonia eutropha H16 presenting a 2-keto-3-desoxy-phosphogluconate aldolase gene-dependent catabolic addiction system for plasmid maintenance when using gluconate or fructose as sole carbon source for synthesis of cyanophycin granule polypeptides. Maximum contents of water-insoluble cyanophycin granule polypeptide and water-soluble cyanophycin granule polypeptide, contributing to 47.5% and 5.8% (w/w) of cell dry matter, respectively, are obtained at the 30-L scale cultivation under optimum conditions
use of a recombinant strain of Ralstonia eutropha H16 presenting a 2-keto-3-desoxy-phosphogluconate aldolase gene-dependent catabolic addiction system for plasmid maintenance when using gluconate or fructose as sole carbon source for synthesis of cyanophycin granule polypeptides. Maximum contents of water-insoluble cyanophycin granule polypeptide and water-soluble cyanophycin granule polypeptide, contributing to 47.5% and 5.8% (w/w) of cell dry matter, respectively, are obtained at the 30-L scale cultivation under optimum conditions