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4.1.2.13: fructose-bisphosphate aldolase

This is an abbreviated version!
For detailed information about fructose-bisphosphate aldolase, go to the full flat file.

Word Map on EC 4.1.2.13

Reaction

D-fructose 1,6-bisphosphate
=
glycerone phosphate
 +
D-glyceraldehyde 3-phosphate

Synonyms

1,6-Diphosphofructose aldolase, 37 kDa major allergen, 41 kDa antigen, ALD, ALDC, aldoA, Aldob, aldolase, aldolase A, aldolase B, aldolase C, aldolase, fructose diphosphate, ALDP, bifunctional fructose-1,6-bisphosphate aldolase/phosphatase, Brain-type aldolase, Ca-FBA-II, CE1, CE2, class I Fba, class I fructose 1,6-bisphosphate aldolase, class I fructose bisphosphate aldolase, class I fructose-1,6-bisphosphate aldolase, class I muscle fructose bis-phosphate aldolase, class II aldolase, class II FBA, class II FBP aldolase, class II fructose 1,6-bisphosphate aldolase, class II fructose bisphosphate aldolase, class II fructose-1,6-bisphosphate aldolase, class IIa fructose 1,6-bisphosphate aldolase, class IIb fructose 1,6-bisphosphate aldolase, ClassII FBP-aldolase, CoFBA1, CoFBA2, CoFBA3, CoFBA4, D-fructose-1,6-bisphosphate aldolase, D-fructose-1,6-bisphosphate D-glyceraldehyde-3-P-lyase, D-fructose-6-phosphate aldolase, Diphosphofructose aldolase, DLF, EC 4.1.2.7, F1,6-P2 aldolase, F1,6P2 aldolase, F16BP aldolase, FBA, FBA class II, FBA-II, Fba1, Fba1p, Fba2, FBA7, FbaA, FbaB, FbaC, FBAld, FbaP, FBP aldolase, FBP aldolase/phosphatase, FBP-aldolase, FBPA, FBPA I, FBPA-1, FBPA/P, FDP aldolase, FPA, Fru-1,6-P2 aldolase, Fru-P2A, Fructoaldolase, fructose 1,6 bisphosphate aldolase, fructose 1,6-bisphosphatase/aldolase, Fructose 1,6-bisphosphate aldolase, fructose 1,6-bisphosphate aldolase/phosphatase, Fructose 1,6-diphosphate aldolase, Fructose 1-monophosphate aldolase, Fructose 1-phosphate aldolase, fructose bis-phosphate aldolase, Fructose bisphosphate aldolase, Fructose diphosphate aldolase, fructose-1,6-biphosphate aldolase, fructose-1,6-bisphosphate (FBP) aldolase/phosphatase, fructose-1,6-bisphosphate aldolase, fructose-1,6-bisphosphate aldolase A, fructose-1,6-bisphosphate aldolase B, fructose-1,6-bisphosphate aldolase class II, fructose-1,6-bisphosphate aldolase/phosphatase, fructose-1,6-bisphosphate muscle aldolase, Fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase, fructose-bisphosphate aldolase A, FSA, fuculose aldolase, heat-induced protein 44, HIP44, IgE-binding allergen, ketose 1-phosphate aldolase, Leishmania aldolase, Liver-type aldolase, MGA3_01355, MJ0400-His6, More, MtFBA, Muscle-type aldolase, Ov-fba-1, OvFBPA-1, Pcal_0111, Phosphofructoaldolase, SMALDO, SSO0286, STK_03180, TgALD1, Tneu_0133, TnFBPAP, Tt-FBPA, zerebrin II, zymohexase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.13 fructose-bisphosphate aldolase

Purification

Purification on EC 4.1.2.13 - fructose-bisphosphate aldolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
5 isoenzymes: A4, A3C1, A2C2, A1C3 and C4
-
95% homogeneity
95% pure
-
ammonium sulfate fractionation, DEAE Sepharose CL-6B resin chromatography, and Resource Q column chromatography
-
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography
ammonium sulfate precipitation, DEAE-Toyopearl 650 S column chromatography, and TSKgel Phenyl 5PW column chromatography
-
anion-exchange column chromatography and gel filtration
-
class I aldolase and class II aldolase
-
class I and class II aldolase
-
class II enzyme
-
CM-Sepharose CL-6B chromatography
Co-TALON column chromatography and Superdex 200 gel filtration
DEAE-52 column chromatography and Superose-12 column chroatography
-
enzyme form I and II
-
enzyme from patients with fructose intolerance
-
glutathione Sepharose 4B, electro elution procedure
glutathione Sepharose column chromatography
HisPur cobalt resin column chromatography
-
HisTrap column chromatography
HisTrap column chromatography and Superdex 75 gel filtration
-
HisTrap column chromatography and Superdex gel filtration
homogeneity
immobilized metal affinity chromatography, Ni2+-IDA affinity chromatography
MonoQ-Sepharose column chromatography and gel filtration
-
near homogeneity
Ni Sepharose 6 column chromatography and Sephadex G-25 gel filtration
-
Ni-nitrilotriacetic acid agarose column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni2+ affinity chromatography
-
Ni2+-NTA column chromatography
nickel affinity chromatography
nickel chelating affinity chromatography and gel filtration
nickel/iminodiacetic acid agarose column chromatography and Superdex 75 gel filtration
-
normal and chimeric enzymes
-
partial
phenyl-Sepharose column chromatography
-
phenyl-Sepharose column chromatography and hydroxylapatite chromatography
-
purification of the recombinant FBPA
recombinant aldolase B and liver enzyme
-
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 25 min, nickel affinity chromatography, and dialysis
recombinant GST-tagged liver enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant protein
recombinant wild-type and mutant enzymes G346S and E206K
Sepharose Q chromatography, Sephacryl 100 gel filtration, and phenyl-Sepharose chromatography
-
simultaneous purification of EC 2.7.1.1, EC 4.1.2.13, EC 5.3.1.1, and EC 2.7.2.3
-