4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Reaction

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.10 (R)-mandelonitrile lyase

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Results	in table
2	Activating Compound
396	AA Sequence
23	Application
1	CAS Registry Number
21	Cloned(Commentary)
10	Cofactor
9	Crystallization (Commentary)
4	General Information
9	General Stability
54	Inhibitors
33	kcat/KM [mM/s]
56	KM Value [mM]
1	Localization
3	Metals/Ions
32	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
14	Organic Solvent Stability
66	Organism
5	Pathway
23	PDB ID
31	pH Optimum
10	pH Range
23	pH Stability
4	pI Value
7	Posttranslational Modification
25	Protein Variants
24	Purification (Commentary)
2	Reaction
5	Reaction Type
63	Reference
27	Source Tissue
19	Specific Activity [micromol/min/mg]
2	Storage Stability
345	Substrates and Products (Substrate)
18	Subunits
95	Synonyms
1	Systematic Name
25	Temperature Optimum [°C]
6	Temperature Range [°C]
43	Temperature Stability [°C]
38	Turnover Number [1/s]

pH Stability

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pH Stability on EC 4.1.2.10 - (R)-mandelonitrile lyase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2.5

3 entries

2.6

Chamberlinius hualienensis

A0A0H5BR52

1 h, about % loss of activity

749169

3 - 9

Rhaphiolepis bibas

B7VF77

60 min, stable

691375

3.5 - 10

Passiflora edulis

A0A1L7NZN4

activities of all the hydroxynitrile lyase are broadly stable over the range from pH 3.5 to 10.0, although the activity of hydroxynitrile lyase expressed in Escherichia coli is reduced to 60% and 70% at pH 7.0 and 10.0, respectively

747548

3.5 - 8

Nandina domestica

-

30°C, 30 min, stable

747326

4

4 entries

4.5

Prunus pedunculata

-

1 h, complete loss of activity

747948

4.75

Arabidopsis thaliana

Q9LFT6

wild-type, half-life 2.2 min, flavin-based fluorescent reporter fusion protein half-life 55-95 min

726741

5

5 entries

5.4

Arabidopsis thaliana

-

half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding

704762

5.5

Chamberlinius hualienensis

A0A0H5BR52

1 h, about 20% loss of activity

749169

6

Arabidopsis thaliana

Q9LFT6

20°C, wild-type, half-life 60 h, surface-modified mutant, half-life 52.2 h

727311

6 - 12

Prunus pedunculata

-

1 h, enzyme is stable

747948

8 - 10

Chamberlinius hualienensis

A0A0H5BR52

1 h, no loss of activity

749169

2.5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity is retained

749351

2.5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity retained

749351

2.5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity retains

749351

4

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity is retained

749351

4

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity retained

749351

4

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 50% of activity retains

749351

4

Prunus pseudoarmeniaca

-

increasing the pH value above 4.0, the yield and ee are dramatically decreased due to the spontaneous chemical decomposition of the product

715946

5

Arabidopsis thaliana

Q9LFT6

20°C, wild-type, half-life 0.16 h, surface-modified mutant, half-life 2.23 h

727311

5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 65% of activity is retained

749351

5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 65% of activity retained

749351

5

Davallia tyermanii

A0A1C9V3R0, A0A1C9V3R4, A0A1C9V3R6, A0A1C9V3S9

8°C, 72 h, more than 65% of activity retains

749351

5

Prunus pedunculata

-

1 h, 25% loss of activity

747948