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4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
 +
benzaldehyde

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

pH Range

pH Range on EC 4.1.2.10 - (R)-mandelonitrile lyase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 4
3.5 - 4.5
-
pH 3.5: about 70% of maximal activity, pH 4.5: about 75% of maximal activity, synthesis of (R)-mandelonitrile
4 - 8
-
4.0 pH: about 60% of maximal activity, pH 8.0: about 50% of maximal activity
4.3 - 5.3
pH 4.3: about 45% of maximal activity, pH 5.3: about 50% of maximal activity
4.5
surface-modified mutant, less than 10% of maximum activity
4.5 - 6
pH 4.5: about 40% of maximal activity, pH 6.0: about 30% of maximal activity
4.75
wild-type, less than 10% of maximum activity
5
-
almost inactive below
5 - 6.5
-
pH 5.0: about 70% of maximal activity, pH 6.5: about 50% of maximal activity, degradation of (R)-mandelonitrile