because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms
1.4 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel)
pH-dependent half-lives: 4.4 min (wild-type enzyme), 5.5 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme), 30.6 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel)
pH-dependent half-lives: 1.5 min (wild-type enzyme), 1.5 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme), 2.1 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel)