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4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
+
benzaldehyde

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

pH Optimum

pH Optimum on EC 4.1.2.10 - (R)-mandelonitrile lyase

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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5
-
at low pH very low amounts of crude enzyme catalyse stereoselective hydroxypivalaldehyde cyanohydrin formation in water based reaction systems
3.5 - 7
-
because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms
4.25
1.4 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel)
4.75
pH-dependent half-lives: 4.4 min (wild-type enzyme), 5.5 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme), 30.6 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel)
4.75 - 5
-
the enzyme shows maximum activity in 0.1 M sodium-citrate buffer having pH 4.75 at 25°C
5 - 6
Prunus sp.
-
-
5.75
surface-modified mutant