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4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
 +
benzaldehyde

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

Specific Activity

Specific Activity on EC 4.1.2.10 - (R)-mandelonitrile lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12.2
wild-type, 25°C, pH 4.75
133.6
134
25°C, pH 4, hydroxynitrile lyase expressed in Pichia pastoris
140
25°C, pH 4, hydroxynitrile lyase mutant N105Q expressed in Pichia pastoris
141
-
pH 5.0, 25°C
2 - 20
pH 5.0, 25°C
220
(R)-HNL after 24.4fold purification, in 0.3 mM citrate buffer, at pH 5.0 and 25°C
227
surface-modified mutant, 25°C, pH 5.75
23.6
surface-modified mutant, 25°C, pH 4.5
54.1
25°C, pH 4, hydroxynitrile lyase expressed in Escherichia coli
661
-
pH 5.5, 50°C
9
(R)-HNL from crude extract, in 0.3 mM citrate buffer, at pH 5.0 and 25°C