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4.1.1.96: carboxynorspermidine decarboxylase

This is an abbreviated version!
For detailed information about carboxynorspermidine decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.96

Reaction

carboxynorspermidine
=
bis(3-aminopropyl)amine
+
CO2

Synonyms

Atu4169, BT_0674, C-NSPD decarboxylase, CANS DC, CANSDC, carboxyspermidine decarboxylase, CasDC, nspC, VC1623

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.96 carboxynorspermidine decarboxylase

Purification

Purification on EC 4.1.1.96 - carboxynorspermidine decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephacryl S-200 gel filtration, and hydroxyapatite column chromatography. The enzyme is greatly stabilized by the addition of both dithiothreitol and EDTA, but not pyridoxal 5'-phosphate, during purification
-
Ni2+ affinity column chromatography and gel filtration
-
nitrilotriacetic acid affinity resin column chromatography
-