4.1.1.86: diaminobutyrate decarboxylase
This is an abbreviated version!
For detailed information about diaminobutyrate decarboxylase, go to the full flat file.
Reaction
Synonyms
DABA aminotransferase/decarboxylase, DABA AT, DABA AT/DC, DABA DC, DABA decarboxylase, DABA-DC, L-2,4-diaminobutyrate aminotransferase/decarboxylase, L-2,4-diaminobutyrate decarboxylase, L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, L-2,4-diaminobutyric acid decarboxylase, More
ECTree
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Purification
Purification on EC 4.1.1.86 - diaminobutyrate decarboxylase
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3660fold to homogeneity by ammonium sulfate fractionation, gel filtration, and two steps of ion exchange chromatography
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native enzyme 168fold by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
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native enzyme 1750fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography
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native enzyme 2150fold to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and two steps of gel filtration
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native enzyme 22.7fold by ammonium sulfate fractionation and anion exchange chromatography
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native enzyme 886fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography
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recombinant enzyme from Escherichia coli strain HB101 to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and hydroxylapatite chromatography