4.1.1.77: 2-oxo-3-hexenedioate decarboxylase

This is an abbreviated version!
For detailed information about 2-oxo-3-hexenedioate decarboxylase, go to the full flat file.

4-OD, 4-oxalocrotonate decarboxylase, 4OD, Decarboxylase, 4-oxalocrotonate, NahK, Oxalocrotonate decarboxylase

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Results	in table
654	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
1	Crystallization (Commentary)
17	Inhibitors
5	kcat/KM [mM/s]
9	Ki Value [mM]
9	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
42	Organism
23	Pathway
9	PDB ID
2	Protein Variants
3	Purification (Commentary)
4	Reaction
1	Reaction Type
17	Reference
2	Source Tissue
2	Specific Activity [micromol/min/mg]
29	Substrates and Products (Substrate)
3	Subunits
13	Synonyms
1	Systematic Name
9	Turnover Number [1/s]

Engineering on EC 4.1.1.77 - 2-oxo-3-hexenedioate decarboxylase

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E109Q

7fold increase in Km-value, 2270fold decrease in kcat, no influence of mutation to vinylpyruvate hydratase activity

E111Q

Km-value comparable to wild-type, 3400fold decrease in kcat no influence of mutation to vinylpyruvate hydratase activity