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4.1.1.72: branched-chain-2-oxoacid decarboxylase

This is an abbreviated version!
For detailed information about branched-chain-2-oxoacid decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.72

Reaction

(3S)-3-methyl-2-oxopentanoate
=
2-methylbutanal
+
CO2

Synonyms

alpha-keto acid decarboxylase, AlsS, BCKA, BCKAD, BCKDC, branched-chain 2-keto acid decarboxylase, branched-chain 2-oxoacid decarboxylase, branched-chain alpha-keto acid decarboxylase, branched-chain alpha-ketoacid dehydrogenase complex, branched-chain keto acid decarboxylase, branched-chain oxo acid decarboxylase, decarboxylase, branched-chain oxo acid, KDC, KdcA, MtKDC, THI3, thiamine metabolism regulatory protein, YDL080c

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.72 branched-chain-2-oxoacid decarboxylase

Molecular Weight

Molecular Weight on EC 4.1.1.72 - branched-chain-2-oxoacid decarboxylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
126700
-
calculated from amino acid sequence
146000
-
gel filtration
160000
165000
Q9HIA4; Q9HIA3
gel filtration
171000
-
with His6-tagged alpha subunits, gel filtration, sucrose density gradient
175000
-
gel filtration
33000
-
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE  
37000
37800
-
2 * 45500, alpha subunit, + 2 * 37800, beta subunit, SDS-PAGE, enzyme forms part of the branched chain alpha-ketoacid dehydrogenase complex
39800
-
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE  
45000
45500
-
2 * 45500, alpha subunit, + 2 * 37800, beta subunit, SDS-PAGE, enzyme forms part of the branched chain alpha-ketoacid dehydrogenase complex
55000
59780
calculated from the deduced amino acid sequence
60900
x * 60900, calculated, x * 62000, SDS-PAGE
61000
4 * 61000, calculated
62000
x * 60900, calculated, x * 62000, SDS-PAGE
73000
-
2 * 73000, gel filtration