4.1.1.7: benzoylformate decarboxylase

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For detailed information about benzoylformate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.7

4.1.1.7

thiamin

putida

thdp-dependent

diphosphate-dependent

carboligation

carboligase

acyloin

r-benzoin

synthesis

s-mandelate

2-keto

biotechnology

Reaction

Synonyms

benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.1 Carboxy-lyases

4.1.1.7 benzoylformate decarboxylase

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Results	in table
1	Activating Compound
1360	AA Sequence
6	Application
1	CAS Registry Number
23	Cloned(Commentary)
36	Cofactor
10	Crystallization (Commentary)
4	General Information
7	General Stability
32	Inhibitors
52	kcat/KM [mM/s]
14	Ki Value [mM]
134	KM Value [mM]
17	Metals/Ions
8	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
112	Organism
10	Pathway
96	PDB ID
13	pH Optimum
2	pH Range
6	pH Stability
96	Protein Variants
26	Purification (Commentary)
2	Reaction
7	Reaction Type
66	Reference
16	Source Tissue
7	Specific Activity [micromol/min/mg]
8	Storage Stability
250	Substrates and Products (Substrate)
13	Subunits
47	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
87	Turnover Number [1/s]

Purification

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Purification on EC 4.1.1.7 - benzoylformate decarboxylase

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5 entries

by Ni-NTA column purification.

Pseudomonas putida

691346

HiTrap Ni2+ chelating column chromatography

Mycolicibacterium smegmatis

747171

large-scale

Pseudomonas putida

4811

Ni-NTA column chromatography

2 entries

Ni-NTA column chromatography and Superdex G200 gel filtration

Pseudomonas putida

P20906

691794

Phenyl Sepharose FF column chromatography and Q Sepharose FF column chromatography

Pseudomonas stutzeri

678422

Q-Sepharose column chromatography and Superdex 200 gel filtration

2 entries

Q-Sepharose column chromatography, Superdex 200 gel filtration

Pseudomonas putida

702796

recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography

Pseudomonas putida

727333

recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography

Pseudomonas putida

728161

recombinant wild-type and mutant enzymes

Pseudomonas putida

P20906

666806