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4.1.1.68: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

This is an abbreviated version!
For detailed information about 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, go to the full flat file.

Reaction

(2Z,4Z)-2-hydroxyhepta-2,4-dienedioate
=
(4Z)-2-oxohept-4-enedioate

Synonyms

2-Hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, 5-Oxopenta-3-ene-1,2,5-tricarboxylate decarboxylase, Decarboxylase, 5-oxopenta-3-ene-1,2,5-tricarboxylate, HHDD isomerase/OPET decarboxylase, HpcE, OPET decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.68 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

Purification

Purification on EC 4.1.1.68 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ammonium sulfate precipitation and gel filtration, additional purification is achieved using DEAE-Sephacel chromatography
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