4.1.1.5: acetolactate decarboxylase

This is an abbreviated version!
For detailed information about acetolactate decarboxylase, go to the full flat file.

ADC, ALD, aldB, ALDC, alpha-Acetolactate decarboxylase, AlsD, BudA1, Decarboxylase, acetolactate

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Results	in table
5	Activating Compound
6236	AA Sequence
6	Application
1	CAS Registry Number
19	Cloned(Commentary)
3	Crystallization (Commentary)
1	General Information
2	General Stability
38	Inhibitors
4	Ki Value [mM]
16	KM Value [mM]
17	Metals/Ions
11	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
314	Organism
6	Pathway
20	PDB ID
16	pH Optimum
7	pH Range
4	pH Stability
2	pI Value
18	Protein Variants
11	Purification (Commentary)
5	Reaction
2	Reaction Type
206	Reference
1	Source Tissue
8	Specific Activity [micromol/min/mg]
1	Storage Stability
142	Substrates and Products (Substrate)
15	Subunits
40	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
6	Temperature Range [°C]
6	Temperature Stability [°C]
4	Turnover Number [1/s]

Purification on EC 4.1.1.5 - acetolactate decarboxylase

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5333fold

ammonium sulfate precipitation, Sephacryl S-300 gel filtration, and Sephacryl S-200 gel filtration

HiTrap DEAE column chromatography and Superdex 200 gel filtration

Ni-NTA column chromatography

recombinant enzyme, expressed in Bacillus subtilis JA222