4.1.1.4: acetoacetate decarboxylase

This is an abbreviated version!
For detailed information about acetoacetate decarboxylase, go to the full flat file.

AAD, AADase, AADC, acetoacetic acid decarboxylase, Acetoacetic decarboxylase, ADC, CP 28/CP 29, Decarboxylase, acetoacetate, Polymyxin MI

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Results	in table
5	Activating Compound
1745	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
3	Crystallization (Commentary)
2	General Information
3	General Stability
42	Inhibitors
1	Ki Value [mM]
13	KM Value [mM]
2	Localization
8	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
46	Organism
7	Pathway
16	PDB ID
7	pH Optimum
3	pH Range
5	pH Stability
15	Protein Variants
8	Purification (Commentary)
6	Reaction
1	Reaction Type
42	Reference
3	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
58	Substrates and Products (Substrate)
9	Subunits
29	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification on EC 4.1.1.4 - acetoacetate decarboxylase

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ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and S-200 gel filtration

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography