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4.1.1.4: acetoacetate decarboxylase

This is an abbreviated version!
For detailed information about acetoacetate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.4

Reaction

acetoacetate
=
acetone
+
CO2

Synonyms

AAD, AADase, AADC, acetoacetic acid decarboxylase, Acetoacetic decarboxylase, ADC, CP 28/CP 29, Decarboxylase, acetoacetate, Polymyxin MI

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.4 acetoacetate decarboxylase

General Stability

General Stability on EC 4.1.1.4 - acetoacetate decarboxylase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
rather easily inactivated by urea, guanidinium chloride and SDS
-
the enzyme dissociates into subunit dimers at pH 8 in 4 M urea solution at low temperature. The subunit dimers can be reassociated to form native, active enzyme by diluting the urea solution with phosphate buffer at pH 6.0 in the presence of dithiothreitol
-