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4.1.1.39: ribulose-bisphosphate carboxylase

This is an abbreviated version!
For detailed information about ribulose-bisphosphate carboxylase, go to the full flat file.

Word Map on EC 4.1.1.39

Reaction

2 3-phospho-D-glycerate + 2 H+ =

D-ribulose 1,5-bisphosphate
+
CO2
 +
H2O

Synonyms

AF_1638, archaeal Rubisco, barley rubisco, Carboxydismutase, CbbL-1, CbbL-2, CbbL1 protein, CbbL2 protein, CbbM, CbbS-1, CbbS-2, D-ribulose 1,5-bisphosphate carboxylase/oxygenase, D-Ribulose 1,5-diphosphate carboxylase, D-Ribulose-1,5-bisphosphate carboxylase, D-ribulose-1,5-bisphosphate carboxylase/oxygenase, Diphosphoribulose carboxylase, form I RubisCO, Form II Rubisco, form III ribulose-1,5-bisphosphate carboxylase/oxygenase, Galdieria Rubisco, green-like type rubisco, LESS17, MbR, Pk-Rubisco, PSS15, PSSU1, Rbc, rbcL, RbcL2, rbcLS, rbcS, RbcS-T, rbcS1, red-type form I RuBisCO, ribulose 1, 5-bisphosphate carboxylase/oxygenase, ribulose 1,5 bisphosphate carboxylase/oxygenase, Ribulose 1,5-bisphosphate carboxylase, Ribulose 1,5-bisphosphate carboxylase-oxygenase, Ribulose 1,5-bisphosphate carboxylase/oxygenase, Ribulose 1,5-diphosphate carboxylase, Ribulose 1,5-diphosphate carboxylase/oxygenase, ribulose bisphosphate carboxylase, Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase-oxygenase, ribulose bisphosphate carboxylase/oxygenase, ribulose bisphosphate carboxylase/oxygenase large subunit, Ribulose diphosphate carboxylase, Ribulose diphosphate carboxylase/oxygenase, ribulose-1, 5-bisphosphate carboxylase/oxygenase, ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase, ribulose-1,5-bisphosphate carboxylase, ribulose-1,5-bisphosphate carboxylase/oxygenase, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, ribulose-bisphosphate carboxylase, ribulose-bisphosphate carboxylase/oxygenase, RLP, Rubisco, RubisCO 1, RuBisCO 2, RubisCO redlike form I, Rubisco small subunit, RuBisCO-like protein, Rubisco-LSU, RubiscoL, RuBP carboxylase, RuBP carboxylase/oxygenase, RuBPC, RuBPcase, RuBPCO, Tk-Rubisco, TK2290, type III ribulose 1,5-bisphosphate carboxylase/oxygenase, type III Rubisco, Water stress responsive proteins 1, 2 and 14

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.39 ribulose-bisphosphate carboxylase

Purification

Purification on EC 4.1.1.39 - ribulose-bisphosphate carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, rate zonal centrifugation using sucrose gradients or Sephacryl S-300 gel filtration (alternatively), and Q Sepharose column chromatography
anion exchange column chromatography and Superdex 200 gel filtration
cell extracts are prepared
-
crude cell extracts bind [14C]2-carboxy-D-arabitinol 1,5-bisphosphate (CABP) to a high molecular mass fraction and CABP-Rubisco is separated by gel filtration chromatography
form I, form II
-
FPLC 15 Q column chromatography and Superose 12 gel filtration
-
His-bind resin column chromatography
-
HiTrap Q HP column chromatography and G25 Sephadex gel filtration
-
Mono-Q column chromatography
-
mutant enzyme
mutant rbc genes transferred into pFL260 for expression in Escherichia coli MV1190
-
partial
partial purification by ammonium sulfate precipitation and Sephadex G-100 gel filtration
-
partial purification of large subunit epsilon N-methyltransferase
-
partial with ammonium sulfate precipitation and ultracentrifugation
partial, cv Caturra
-
partially, hybrid enzymes
-
PD-10 gel filtration
-
polyethylene glycol precipitation and Protein-Pak Q column chromatography
-
Q sepharose column chromatography
Q-Sepharose column chromatography
Q-Sepharose column chromatography, Superose 12 gel filtration, and phenyl-Sepharose column chromatography
-
recombinant enzyme
-
Seppro IgY-Rusbico Spin affinity column chromatography
-
streptomycin and ammonium sulfate precipitations
Superdex 200 gel filtration
Superdex-200 Hiload 16/60 gel filtration and MonoQ anion exchange column chromatography
-
to identify the S-nitrosylated proteins, neutravidin affinity chromatography is used to purify biotinylated proteins from Kalanchoe pinnata leaf extracts
using a Econo-Pac 10DG desalting column and filtration
using a HiTrapQ DEAE sepharose and a MonoQ column
Thermosynechococcus vestitus
-
using column chromatography
using metal affinity chromatography
-
using Ni-NTA chromatography
using Ni-NTA chromatography and ion-exchange chromatography
-
using Q-sepharose anion exchange chromatography and gel filtration
-
wild-type and mutant proteins