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4.1.1.39: ribulose-bisphosphate carboxylase

This is an abbreviated version!
For detailed information about ribulose-bisphosphate carboxylase, go to the full flat file.

Word Map on EC 4.1.1.39

Reaction

2 3-phospho-D-glycerate + 2 H+ =

D-ribulose 1,5-bisphosphate
+
CO2
 +
H2O

Synonyms

AF_1638, archaeal Rubisco, barley rubisco, Carboxydismutase, CbbL-1, CbbL-2, CbbL1 protein, CbbL2 protein, CbbM, CbbS-1, CbbS-2, D-ribulose 1,5-bisphosphate carboxylase/oxygenase, D-Ribulose 1,5-diphosphate carboxylase, D-Ribulose-1,5-bisphosphate carboxylase, D-ribulose-1,5-bisphosphate carboxylase/oxygenase, Diphosphoribulose carboxylase, form I RubisCO, Form II Rubisco, form III ribulose-1,5-bisphosphate carboxylase/oxygenase, Galdieria Rubisco, green-like type rubisco, LESS17, MbR, Pk-Rubisco, PSS15, PSSU1, Rbc, rbcL, RbcL2, rbcLS, rbcS, RbcS-T, rbcS1, red-type form I RuBisCO, ribulose 1, 5-bisphosphate carboxylase/oxygenase, ribulose 1,5 bisphosphate carboxylase/oxygenase, Ribulose 1,5-bisphosphate carboxylase, Ribulose 1,5-bisphosphate carboxylase-oxygenase, Ribulose 1,5-bisphosphate carboxylase/oxygenase, Ribulose 1,5-diphosphate carboxylase, Ribulose 1,5-diphosphate carboxylase/oxygenase, ribulose bisphosphate carboxylase, Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase-oxygenase, ribulose bisphosphate carboxylase/oxygenase, ribulose bisphosphate carboxylase/oxygenase large subunit, Ribulose diphosphate carboxylase, Ribulose diphosphate carboxylase/oxygenase, ribulose-1, 5-bisphosphate carboxylase/oxygenase, ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase, ribulose-1,5-bisphosphate carboxylase, ribulose-1,5-bisphosphate carboxylase/oxygenase, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, ribulose-bisphosphate carboxylase, ribulose-bisphosphate carboxylase/oxygenase, RLP, Rubisco, RubisCO 1, RuBisCO 2, RubisCO redlike form I, Rubisco small subunit, RuBisCO-like protein, Rubisco-LSU, RubiscoL, RuBP carboxylase, RuBP carboxylase/oxygenase, RuBPC, RuBPcase, RuBPCO, Tk-Rubisco, TK2290, type III ribulose 1,5-bisphosphate carboxylase/oxygenase, type III Rubisco, Water stress responsive proteins 1, 2 and 14

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.39 ribulose-bisphosphate carboxylase

Cloned

Cloned on EC 4.1.1.39 - ribulose-bisphosphate carboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an antisense rbcS gene vector is constructed, the fragment is cloned into pUC118 and subsequently into pTGV2
cDNA and tobacco gene cloned and sequenced
-
cDNA cloned and sequenced
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli as a His-tagged fusion protein and in tobacco chloroplasts
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells
-
expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells and Rosetta2(DE3)pLysS cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli strain BL21
-
expressed in Escherichia coli XL1-Blue cells
expression in Chlamydomonas reinhardtii
DV157962
expression in Escherichia coli
expression of chimeric enzyme hybrides of Nicotiana tabacum, Helianthus annuus, Synechococcus sp.
expression of heterologuous enzymes of Anacystis nidulans, Alcaligenes eutrophus, Rhodobacter sphaeroides in Escherichia coli
-
gene cbbL, form IA RuBisCo, and gene cbbM, form II RuBisCo, are cloned using the TopoTA cloning kit and subsequently sequenced
gene rbcL for the large subunit specifically mutated by transforming the chloroplast genome
-
hybrid enzymes synthesized in Escherichia coli with synthetic genes for small subunits of rice, tobacco, and wheat
-
into the pGEM-T easy vector and subsequently into pET-22b+ for expression in Escherichia coli BL21DE3 cells
A8C9T6; A8C9T7, A8C9T8; A8C9T9
into the pUC18 plasmid for expression in Escherichia coli DH5alpha cells
Thermosynechococcus vestitus
-
large subunit expressed in Nicotiana tabacum
partial reconstruction of the calvin cycle in Escherichia coli and its application in the directed evolution of RuBisCO hypermorphs
-
plasmid harboring gene Pk-rbc1 transformed into Escherichia coli BL21(DE3)
-
rbc gene introduced in Nicotiana tabacum
rbc gene introduced into Nicotiana tabacum
-
rbcLS genes, Rhodobacter capsulatus as host
-
rbcS expressed in Escherichia coli, together with rbcL from cyanobacteria generates functional holoenzyme
RbcS is expressed in Oryza sativa cultivar Nipponbare
-
recombinantly expressed in Escherichia coli
replacement of the enzyme with the dimeric Rhodospirillum rubrum enzyme by biolistic plastid transformation
-
small subunit gene rbcS expressed in Escherichia coli, together with the gene encoding the large subunit, rbcL from cyanobacteria, generates a functional holoenzyme
the rbcS cDNA is expressed in Chlamydomonas reinhardtii rbcS deletion mutant T60-3 by fusing it to a Chlamydomonas rbcS transit peptide sequence engineered to contain rbcS introns
transformed into Escherichia coli, transforming of a deficient Chlamydomonas strain
-