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4.1.1.31: phosphoenolpyruvate carboxylase

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxylase, go to the full flat file.

Word Map on EC 4.1.1.31

Reaction

phosphate
+
oxaloacetate
=
phosphoenolpyruvate
+
HCO3-

Synonyms

archaeal-type phosphoenolpyruvate carboxylase, AT1G53310, atPEPC, Atppc1, Atppc2, Atppc3, Atppc4, bacterial-type PEPC, bacterial-type phosphoenolpyruvate carboxylase, BPTC, BTPC, C4 PEPC, C4-PEPC, Carboxylase, phosphopyruvate (phosphate), Class-1 PEPC, Class-2 PEPC, CP21, CP28, CP46, CrPpc1, CrPpc2, Hvpepc3, Hvpepc4, Hvpepc5, Ljpepc1, NCgl1523, OJ1484_G09.129-1, Osppc2a, Osppc4, PEOC, PEP carboxylase, PEP-carboxylase, PEPC, PEPC(p102)kinase, PEPC1, PEPC2, PEPC3, PEPC7, PepcA, PEPCase, PEPCase1, PEPCK, PEPK, phosphoenol pyruvate carboxylase, phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase, Phosphoenolpyruvic carboxylase, photosynthetic phosphoenolpyruvate carboxylase, plant-type phosphoenolpyruvate carboxylase, PPC, PPC1, PPC2, PPC2a, PPC3, PPC4, PpcA, PpcA1, PPCK1, PPCK2, PTPC, S.minutum PEPC1, Sb02g021090, Sb04g008720, SORBI_3007G106500, SSO2256, SvPEPC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.31 phosphoenolpyruvate carboxylase

Purification

Purification on EC 4.1.1.31 - phosphoenolpyruvate carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms: a night form and a day form
-
a dark-adapted enzyme form and a light-adapted enzyme form
-
a high-molecular-mass isoform, PEPC2, and a low-molecular-mass isoform, PEPC1
-
affinity chromatography and gel filtration
affinity purification
agarose resin column chromatography
-
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Fractogel EMD DEAE-650 colum chromatography, Mono Q column chromatography, and Superdex-200 gel filtration
-
butyl-Sepharose column chromatography
-
His-Trap FF or HP column chromatography
isoenzyme PC-I and PC-II
-
isoform-specific purification of CrPpc1 and CrPpc2 is carried out by affinity-purification of using CrPpc1 and CrPpc2 peptide antibodies
-
large scale purification
-
Ni-NTA column chromatography, Superose-6 gel filtration, and Superdex 200 gel filtration
Ni2+ affinity resin column chromatography, Superdex 200 gel filtration, and Superose-6 gel filtration
-
Ni2+ affinity resin column chromatography, Superdex-200 gel filtration, and Superose-6 gel filtration
-
Ni2+-nitrilotriacetic acid column chromatography
-
partial
partial, 2 isoenzymes: I and II
-
partial, 2 isoforms: PEPC1 and PEPC2
-
recombinant
-
recombinant enzyme
-
recombinant His-tagged Ppc1, expression in Escherichia coli
recombinant His-tagged Ppc2, expression in Escherichia coli
Sephadex G-25 gel filtration
-
wild type and mutant enzymes S8C, and S8D
Sorghum sp.
-