4.1.1.31: phosphoenolpyruvate carboxylase

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxylase, go to the full flat file.

Reaction

Synonyms

archaeal-type phosphoenolpyruvate carboxylase, AT1G53310, atPEPC, Atppc1, Atppc2, Atppc3, Atppc4, bacterial-type PEPC, bacterial-type phosphoenolpyruvate carboxylase, BPTC, BTPC, C4 PEPC, C4-PEPC, Carboxylase, phosphopyruvate (phosphate), Class-1 PEPC, Class-2 PEPC, CP21, CP28, CP46, CrPpc1, CrPpc2, Hvpepc3, Hvpepc4, Hvpepc5, Ljpepc1, NCgl1523, OJ1484_G09.129-1, Osppc2a, Osppc4, PEOC, PEP carboxylase, PEP-carboxylase, PEPC, PEPC(p102)kinase, PEPC1, PEPC2, PEPC3, PEPC7, PepcA, PEPCase, PEPCase1, PEPCK, PEPK, phosphoenol pyruvate carboxylase, phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase, Phosphoenolpyruvic carboxylase, photosynthetic phosphoenolpyruvate carboxylase, plant-type phosphoenolpyruvate carboxylase, PPC, PPC1, PPC2, PPC2a, PPC3, PPC4, PpcA, PpcA1, PPCK1, PPCK2, PTPC, S.minutum PEPC1, Sb02g021090, Sb04g008720, SORBI_3007G106500, SSO2256, SvPEPC

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.31 phosphoenolpyruvate carboxylase

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Results	in table
107	Activating Compound
18813	AA Sequence
5	Application
1	CAS Registry Number
46	Cloned(Commentary)
24	Cofactor
9	Crystallization (Commentary)
10	Expression
45	General Information
16	General Stability
61	IC50 Value
321	Inhibitors
4	kcat/KM [mM/s]
10	Ki Value [mM]
198	KM Value [mM]
40	Localization
123	Metals/Ions
119	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
511	Organism
23	Pathway
41	PDB ID
67	pH Optimum
15	pH Range
3	pH Stability
2	pI Value
49	Posttranslational Modification
50	Protein Variants
72	Purification (Commentary)
2	Reaction
3	Reaction Type
243	Reference
185	Source Tissue
154	Specific Activity [micromol/min/mg]
16	Storage Stability
252	Substrates and Products (Substrate)
70	Subunits
314	Synonyms
1	Systematic Name
35	Temperature Optimum [°C]
6	Temperature Range [°C]
62	Temperature Stability [°C]
18	Turnover Number [1/s]

General Stability

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General Stability on EC 4.1.1.31 - phosphoenolpyruvate carboxylase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

10 mM Mg2+ stabilizes the enzyme against cold inactivation. At low Mg2+ concentrations, 4 mM, the enzyme is strongly protected by phosphoenolpyruvate, glucose-6-phosphate, and, partially, by L-malate

Atriplex halimus

-

645465

desalted enzyme, in Tris buffer, pH 7.5, room temperature, half-life is 3-4 h

Spinacia oleracea

-

4288

glycerol increases stability

Amaranthus hypochondriacus

-

656910

inactivated by trypsin under non-catalytic conditions, resulting in a truncated enzyme having molecular mass of about 80000 Da. In presence of substrate very little inactivation is observed although the subunit molecular mass is reduced to 90000 Da

Zea mays

-

655705

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

5 entries

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

5 entries

retains full activity after exposure to 0.5 M guanidine-HCl at 30°C for 20 h

Thermostichus vulcanus

P0A3X6

656978

thiol protecting agents stabilize during purification

Kalanchoe fedtschenkoi

-

4292

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

Panicum milioides

-

4313

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

Panicum schenckii

-

4313

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

Steinchisma decipiens

-

4313

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

Steinchisma laxum

-

4313

malate, 10 mM, protects from inactivation at 55�C, at pH 5.5, but not at pH 8.3

Coleataenia prionitis

-

4313

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

Panicum milioides

-

4313

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

Panicum schenckii

-

4313

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

Steinchisma decipiens

-

4313

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

Steinchisma laxum

-

4313

phosphoenolpyruvate, 5 mM, stabilizes the enzyme at 37�C in plant extract

Coleataenia prionitis

-

4313