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4.1.1.18: lysine decarboxylase

This is an abbreviated version!
For detailed information about lysine decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.18

Reaction

L-lysine
=
cadaverine
+
CO2

Synonyms

AsLdc, CadA, constitutive LDCc, constitutive lysine decarboxylase, DesA, EcLDCc, ECORLD, gtLDC, inducible lysine decarboxylase, L-Lys-OD, L-lysine decarboxylase, LDC, ldcC, LdcI, LdcI/CadA, LysA, lysine decarboxylase, MaLDC, multimeric lysine decarboxylase, SrLDC, VSAL_I2491

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.18 lysine decarboxylase

Cloned

Cloned on EC 4.1.1.18 - lysine decarboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Corynebacterium glutamicum ATCC 13032
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
expression in Escherichia coli
-
gene cadA from strain V02-64, DNA and amino acid sequence, and gene cluster and intergenic region determination and analysis, subcloning in Escherichia coli, quantitative analysis of lysine decarboxylase expression levels dependent on acid conditions at pH 4.0-7.5, primer extension analysis, the gene encoding the enzyme is located in an acid-induced operon, overview, cadA mRNA exhibited about 40fold greater expression at pH 5.0 than at pH 5.5, in cells exposed to pH 7.5, the cadA transcript shows the largest quantity under all conditions, the amount of cadA single transcript is nearly 100fold higher than the polycistronic cadBA transcript for each pH condition
gene cadA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cadA, encoded in the cadBA operon, also coding for a lysine-cadaverine antiporter, CadB, expression of cadA in Escherichia coli strain S17-1 and S17-1lambdapir, transcription of cadBA in a pH-dependent manner, overview
-
gene cadA, recombinant enzyme expression in Escherichia coli strain XL-1 Blue
gene cadA, recombinant expression of His-tagged wild-type and T88 mutants in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene cadA, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
gene cadA, recombinant induced expression of lysine decarboxylase in Escherichia coli strain BL21(DE3) for the high-level production of cadaverine from industrial grade L-lysine
gene cadA, sequence comparisons and phylogenetic analysis, recombinant functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), the activity of purified CadA from Klebsiella pneumoniae is lower than that of CadA from Escherichia coli, the activity of Klebsiella pneumoniae CadA in whole-cell bioconversion is comparable to that of Escherichia coli CadA, resulting in 90% lysine conversion of L-lysine to cadaverine with cofactor pyridoxal 5'-phosphate
gene gtLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene ldc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the soluble His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time reverse transcription PCR enzyme expression analysis
gene ldc, recombinant expression of codon-optimized enzyme in Escherichia coli K-12 resulting in strain LN20, optimizing the sequence located in the region of the ribosome-binding site in order to enhance translation efficiency further improves the system, subcloning in Escherichia coli TOP10 cells. Batch and fed-batch production of Klebsiella oxytoca lysine decarboxylase from several modifed strains, overview
-
gene ldc, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109/pTrc99a-ldc2-41
-
gene ldc, recombinant expression of wild-type enzyme and codon-optimized enzyme in Escherichia coli K-12 resulting in strains LN18 and LN20, respectively. Batch and fed-batch production of Klebsiella oxytoca lysine decarboxylase from several modifed strains, overview
-
gene ldcC, recombinant constitutive expression of lysine decarboxylase in Escherichia coli strain BL21(DE3) for the high-level production of cadaverine from industrial grade L-lysine
gene ldcC, recombinant expression in Escherichia coli strain BL21(DE3), optimization of conditions for enzyme overexpression, e.g. with respect to pH, cadaverine concentration, and IPTG concentration, overview
gene ldcC, recombinant overexpression in Escherichia coli strain BL21(DE3) in inclusion bodies with 3-10 mg/ml protein in batch cultivation. The aggregated enzyme EcLDCc-CatIBs is catalytically active
gene ldcC, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ldcI, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
gene lysA, recombinant enzyme overexpression in Escherichia coli strain LN18
gene Srldc, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)