4.1.1.102: phenacrylate decarboxylase

This is an abbreviated version!
For detailed information about phenacrylate decarboxylase, go to the full flat file.

Reaction

Synonyms

FADase, Fdc, FDC1, ferulic acid decarboxylase, PAD, PAD1, phenolic acid decarboxylase, ubiD, YDR539W

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.102 phenacrylate decarboxylase

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Results	in table
4	Activating Compound
738	AA Sequence
9	Application
13	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
5	General Information
8	Inhibitors
22	kcat/KM [mM/s]
11	KM Value [mM]
2	Localization
8	Metals/Ions
8	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
36	Organism
2	Pathway
53	PDB ID
5	pH Optimum
4	pH Range
2	pH Stability
1	pI Value
43	Protein Variants
4	Purification (Commentary)
3	Reaction
24	Reference
3	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
7	Subunits
26	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
3	Temperature Range [°C]
1	Temperature Stability [°C]
9	Turnover Number [1/s]

Engineering

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of the Enzyme Summary Page.

Engineering on EC 4.1.1.102 - phenacrylate decarboxylase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D112N

Enterobacter sp.

C6F3U5

121% of wild-type activity

733201

E134A

Enterobacter sp.

C6F3U5

mutation decreases the enzyme activity by more than 60%

735040

F95A

Enterobacter sp.

C6F3U5

120% of wild-type activity

733201

F95A/D112N

Enterobacter sp.

C6F3U5

395% of wild-type activity

733201

F95A/S157I

Enterobacter sp.

C6F3U5

218% of wild-type activity

733201

F95A/V151I

Enterobacter sp.

C6F3U5

333% of wild-type activity

733201

F95E

Enterobacter sp.

C6F3U5

26% of wild-type activity

733201

F95I

Enterobacter sp.

C6F3U5

148% of wild-type activity

733201

F95I/D112N

Enterobacter sp.

C6F3U5

418% of wild-type activity

733201

F95I/S157I

Enterobacter sp.

C6F3U5

299% of wild-type activity

733201

F95I/V151I

Enterobacter sp.

C6F3U5

323% of wild-type activity

733201

F95K

Enterobacter sp.

C6F3U5

18% of wild-type activity

733201

F95L

Enterobacter sp.

C6F3U5

188% of wild-type activity

733201

F95L/D112N

Enterobacter sp.

C6F3U5

485% of wild-type activity

733201

F95L/D112N/S157I

Enterobacter sp.

C6F3U5

430% of wild-type activity

733201

F95L/D112N/V151I

2 entries

F95L/S157I

Enterobacter sp.

C6F3U5

528% of wild-type activity

733201

F95L/V151I

Enterobacter sp.

C6F3U5

746% of wild-type activity

733201

F95L/V151I/S157I

Enterobacter sp.

C6F3U5

484% of wild-type activity

733201

F95Q

Enterobacter sp.

C6F3U5

48% of wild-type activity

733201

F95R

Enterobacter sp.

C6F3U5

19% of wild-type activity

733201

S157I

Enterobacter sp.

C6F3U5

171% of wild-type activity

733201

V151I

Enterobacter sp.

C6F3U5

174% of wild-type activity

733201

W25A

Enterobacter sp.

C6F3U5

mutation decreases the enzyme activity by more than 95%

735040

Y21A

Enterobacter sp.

C6F3U5

mutation abolishes the enzyme activity completely

735040

Y27A

Enterobacter sp.

C6F3U5

mutation abolishes the enzyme activity completely

735040

D112N

Enterobacter sp. Px6-4

-

121% of wild-type activity

-

E134A

Enterobacter sp. Px6-4

-

mutation decreases the enzyme activity by more than 60%

-

F95A

Enterobacter sp. Px6-4

-

120% of wild-type activity

-

F95L

Enterobacter sp. Px6-4

-

188% of wild-type activity

-

F95L/D112N/V151I

2 entries

W25A

Enterobacter sp. Px6-4

-

mutation decreases the enzyme activity by more than 95%

-

Y21A

Enterobacter sp. Px6-4

-

mutation abolishes the enzyme activity completely

-

Y27A

Enterobacter sp. Px6-4

-

mutation abolishes the enzyme activity completely

-

M57A

Meyerozyma guilliermondii

G3XHW5

almost complete loss of activity

733069

M57L

Meyerozyma guilliermondii

G3XHW5

mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate

733069

M57T

Meyerozyma guilliermondii

G3XHW5

almost complete loss of activity

733069

M57A

Meyerozyma guilliermondii ATCC 9058

-

almost complete loss of activity

-

M57L

Meyerozyma guilliermondii ATCC 9058

-

mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate

-

M57T

Meyerozyma guilliermondii ATCC 9058

-

almost complete loss of activity

-

E285A

Saccharomyces cerevisiae

Q03034

loss of catalytic activity

733178

F95L/D112N/V151I

Enterobacter sp.

C6F3U5

1354% of wild-type activity

733201

F95L/D112N/V151I

Enterobacter sp.

C6F3U5

approximately 34fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid, possibly due to formation of a compact active site compared with that of the wild-type

733201

F95L/D112N/V151I

Enterobacter sp. Px6-4

-

1354% of wild-type activity

-

F95L/D112N/V151I

Enterobacter sp. Px6-4

-

approximately 34fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid, possibly due to formation of a compact active site compared with that of the wild-type

-