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3.9.1.3: phosphohistidine phosphatase

This is an abbreviated version!
For detailed information about phosphohistidine phosphatase, go to the full flat file.

Word Map on EC 3.9.1.3

Reaction

a [protein]-N-phospho-L-histidine
+
H2O
=
a [protein]-L-histidine
 +
phosphate

Synonyms

14 kDa phosphohistidine phosphatase, 14-kDa phosphohistidine phosphatase, 16 kDa protein histidine phosphatase, histidine protein phosphatase, LHPP, PGAM5, phospho-histidine phosphatase, phosphohistidine phosphatase, phosphohistidine phosphatase 1, phosphohistidine phosphatase I, phosphopolyhistidine phosphatase, PHP, PHP14, PHPT-1, PHPT1, PHPTI, PP1, PP2A, protein histidine phosphatase, protein histidine phosphatase 1, protein-histidine phosphatase, SixA

ECTree

     3 Hydrolases
         3.9 Acting on phosphorus-nitrogen bonds
             3.9.1 Acting on phosphorus-nitrogen bonds (only sub-subclass identified to date)
                3.9.1.3 phosphohistidine phosphatase

Specific Activity

Specific Activity on EC 3.9.1.3 - phosphohistidine phosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0028
-
crude enzyme, at pH 7.5 and 30°C
0.06
cell lysate, mutant enzyme R45A, pH and temperature not specified in the publication
0.2
purified mutant enzyme R45A, pH and temperature not specified in the publication
0.3
cell lysate, mutant enzyme R78A, pH and temperature not specified in the publication
0.9
purified mutant enzyme R78A, pH and temperature not specified in the publication
1.4
purified mutant enzyme H81A, pH and temperature not specified in the publication
1.8
purified wild type enzyme, pH and temperature not specified in the publication
2.72
-
after 971fold purification, at pH 7.5 and 30°C