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3.9.1.3: phosphohistidine phosphatase

This is an abbreviated version!
For detailed information about phosphohistidine phosphatase, go to the full flat file.

Word Map on EC 3.9.1.3

Reaction

a [protein]-N-phospho-L-histidine
+
H2O
=
a [protein]-L-histidine
 +
phosphate

Synonyms

14 kDa phosphohistidine phosphatase, 14-kDa phosphohistidine phosphatase, 16 kDa protein histidine phosphatase, histidine protein phosphatase, LHPP, PGAM5, phospho-histidine phosphatase, phosphohistidine phosphatase, phosphohistidine phosphatase 1, phosphohistidine phosphatase I, phosphopolyhistidine phosphatase, PHP, PHP14, PHPT-1, PHPT1, PHPTI, PP1, PP2A, protein histidine phosphatase, protein histidine phosphatase 1, protein-histidine phosphatase, SixA

ECTree

     3 Hydrolases
         3.9 Acting on phosphorus-nitrogen bonds
             3.9.1 Acting on phosphorus-nitrogen bonds (only sub-subclass identified to date)
                3.9.1.3 phosphohistidine phosphatase

Purification

Purification on EC 3.9.1.3 - phosphohistidine phosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Blue Sepharose column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Mono-Q column chromatography, and Sephacryl S-100 gel filtration
DE-52 cellulose column chromatography
-
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration
expression in Escherichia coli
HiTrap column chromatography and Superdex 75 gel filtration
Mono Q column chromatography
MonoQ column chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA agarose column chromatography, and gel filtration
-
Ni-NTA column chromatography
Ni2+-NTA agarose column chromatography
-