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3.8.1.9: (R)-2-haloacid dehalogenase

This is an abbreviated version!
For detailed information about (R)-2-haloacid dehalogenase, go to the full flat file.

Word Map on EC 3.8.1.9

Reaction

(R)-2-haloacid
+
H2O
=
(S)-2-hydroxyacid
 +
halide

Synonyms

2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, D-2-haloacid dehalogenase, D-2-MCPA dehalogenase, D-DEX, D-specific dehalogenase, D-specific mono chloro propionoic acid dehalogenase, DEH138, DehD, DehIII, HadD AJ1, R-2-haloacid dehalogenase

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.9 (R)-2-haloacid dehalogenase

Engineering

Engineering on EC 3.8.1.9 - (R)-2-haloacid dehalogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D205E
activity with D-2-chloropropionate is 10.1% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 13fold higher compared to mutant enzyme
D205N
F281A
activity with D-2-chloropropionate is 54.7% compared to activity of the wild-type enzyme
G50A
activity with D-2-chloropropionate is 5.1% compared to activity of the wild-type enzyme
I52G
activity with D-2-chloropropionate is 1.5% compared to activity of the wild-type enzyme
L285I
activity with D-2-chloropropionate is 24.4% compared to activity of the wild-type enzyme
L288A
the mutant enzyme is active toward L-enantiomers. The catalytic efficiency is less than 30% of mutant enzyme L288I
L288I
L288V
kcat/Km of the L288V mutant displays over 80% of that of the L288I mutant
M284C
activity with D-2-chloropropionate is 7.0% compared to activity of the wild-type enzyme
M284F
mutant enzyme shows no enantioselective changes in contrast to the wild-type enzyme
N131D
activity with D-2-chloropropionate is 4.4% compared to activity of the wild-type enzyme
N203A
N203S
activity with D-2-chloropropionate is 0.4% compared to activity of the wild-type enzyme
S204A
activity with D-2-chloropropionate is 4.9% compared to activity of the wild-type enzyme
S204T
activity with D-2-chloropropionate is 41.8% compared to activity of the wild-type enzyme
V51F
activity with D-2-chloropropionate is 36.6% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 4fold higher compared to mutant enzyme
W48A
activity with D-2-chloropropionate is 0.2% compared to activity of the wild-type enzyme
Y134F
activity with D-2-chloropropionate is 0.1% compared to activity of the wild-type enzyme
D205N
-
no activity with D-2-chloropropionate
-
G50A
-
activity with D-2-chloropropionate is 5.1% compared to activity of the wild-type enzyme
-
N131D
-
activity with D-2-chloropropionate is 4.4% compared to activity of the wild-type enzyme
-
S204A
-
activity with D-2-chloropropionate is 4.9% compared to activity of the wild-type enzyme
-
W48A
-
activity with D-2-chloropropionate is 0.2% compared to activity of the wild-type enzyme
-
R134A
site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates
Y135A
site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates