3.8.1.3: haloacetate dehalogenase
This is an abbreviated version!
For detailed information about haloacetate dehalogenase, go to the full flat file.
Word Map on EC 3.8.1.3
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3.8.1.3
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dehalogenation
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haloalkane
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xanthobacter
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autotrophicus
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had-like
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moraxella
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defluorination
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l-2-haloacids
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chloroacetate
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phospho1
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2-haloacid
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beta-phosphoglucomutase
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1,2-dichloroethane
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agriculture
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environmental protection
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analysis
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degradation
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biotechnology
- 3.8.1.3
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dehalogenation
- haloalkane
- xanthobacter
- autotrophicus
-
had-like
- moraxella
-
defluorination
- l-2-haloacids
- chloroacetate
- phospho1
-
2-haloacid
- beta-phosphoglucomutase
- 1,2-dichloroethane
- agriculture
- environmental protection
- analysis
- degradation
- biotechnology
Reaction
Synonyms
Fac-DEX, FAc-Dex FA1, FAc-Dex H1, FAcD, fluoroacetate dehalogenase, fluoroacetate-specific defluorinase, haloacid dehalogenase, magnesium-dependent acid phosphatase-1, MDP-1, monohaloacetate dehalogenase, monohaloacetate halidohydrolase, More, PH0459
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General Information
General Information on EC 3.8.1.3 - haloacetate dehalogenase
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physiological function
additional information
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fluoroacetate dehalogenase catalyzes degradation of the environmentally toxic fluoroacetate through a two step process initiated by an SN2 reaction in which the aspartate residue performs a nucleophilic attack on the carbon bonded to the fluorine, the second step is hydrolysis that releases the product as glycolate
physiological function
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the degradation of haloacid is mediated by the production of the inducible dehalogenase. Presence of distinct inducible acetate- and monochloroacetate-transport system in MBA4. Determination of relative acetate- and monochloroacetate-uptake activities of MBA4 grown in various substrates and inhibition of acetate- and monochloroacetate-uptake by other solutes, overview
physiological function
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the degradation of haloacid is mediated by the production of the inducible dehalogenase. Presence of distinct inducible acetate- and monochloroacetate-transport system in MBA4. Determination of relative acetate- and monochloroacetate-uptake activities of MBA4 grown in various substrates and inhibition of acetate- and monochloroacetate-uptake by other solutes, overview
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the active site is formed by Phe34, Asp104, Arg105, Arg108, Asp128, His271, and Phe272 of the core domain, as well as of Tyr147, His149, Trp150, and Tyr212 of the cap domain, structure analysis, overview
additional information
the enzyme active site is composed of Asp104, Arg105, Arg108, His149, Trp150, Tyr212, and His271, environments of the tryptophan residues are different between the fluoroacetate and chloroacetate-bound enzymes, active site docking simulations and QM/MM optimizations, QM/MM calculations for the defluorination of fluoroacetate, overview
additional information
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density functional theory calculations of the SN2 initiation reaction modeled through the interaction between the substrate and the propionate anion as the nucleophile, the reaction energy is modulated by the degree of stabilization of the fluoride anion formed after the SN2 reaction. The activation of this catalytic step is associated to the interaction of the halogen with residues His155, Trp156, and Tyr219, which may facilitate the Calpha-F bond dissociation