3.8.1.10: 2-haloacid dehalogenase (configuration-inverting)

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Reaction

Synonyms

2-HAD, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, DL DEX 113, DL-2-haloacid dehalogenase, DL-2-haloacid dehalogenase (inversion of configuration), DL-2-haloacid halidohydrolase (inversion of configuration), DL-DEX, DL-DEX 113, DL-DEX Mb, DL-DEXi, L-2-haloacid dehalogenase, L-DEX, L-DEX YL, ps-2-HAD

ECTree

     3 Hydrolases

         3.8 Acting on halide bonds

             3.8.1 In carbon-halide compounds

                3.8.1.10 2-haloacid dehalogenase (configuration-inverting)

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Results	in table
12	AA Sequence
1	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
4	Expression
4	General Information
25	Inhibitors
4	Ki Value [mM]
16	KM Value [mM]
7	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
122	Organism
12	PDB ID
7	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
21	Protein Variants
6	Purification (Commentary)
4	Reaction
1	Reaction Type
45	Reference
1	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
147	Substrates and Products (Substrate)
12	Subunits
27	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]

Crystallization

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Crystallization on EC 3.8.1.10 - 2-haloacid dehalogenase (configuration-inverting)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant native and selenomethionine-labeled wild-type enzyme and recombinant D194N enzyme mutant complexed with inhibitor 2-bromo-2-methylpropionate, 15 mg/mL protein in 10 mM Hepes-NaOH, pH 7.5, is mixed with 3 M sodium formate, 5% glycerol v/v, as the reservoir solution, X-ray diffraction structure determination and analysis at 1.7-2.7 A resolution

Methylobacterium sp.

A6BM74

733212

purified enzyme, sitting drop vapour diffusion method, mixing of equal volumes of 9 mg/ml protein in 50 mM Tris, pH 8.0, and 25 mM NaCl, and of reservoir solution consisting of 0.2 M MgCl2 hexahydrate, 20-26% PEG 4000, 0.1 M Tris, pH 8.5, and equilibration against 0.1 ml of reservoir solution, 4°C, overnight, soaking of crystals in KI, X-ray diffraction structure determination and analysis at 1.98-2.0 A resolution, single-wavelength anomalous dispersion method, modeling

Pseudomonas syringae

Q88B12

732999