3.7.1.9: 2-hydroxymuconate-6-semialdehyde hydrolase

This is an abbreviated version!
For detailed information about 2-hydroxymuconate-6-semialdehyde hydrolase, go to the full flat file.

Word Map on EC 3.7.1.9

3.7.1.9

catechols

extradiol

sphingomonas

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate

2-hydroxy-6-oxohepta-2,4-dienoate

lignin-related

4-hydroxy-2-oxovalerate

xylf

paucimobilis

5-carboxyvanillate

4-oxalocrotonate

m-cresol

isopropylbenzene

toluate

3-methylcatechol

molecular biology

Reaction

Synonyms

1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase, 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase, 2-hydroxymuconate semialdehyde hydrolase, 2-hydroxymuconate-semialdehyde hydrolase, 2-hydroxymuconic semialdehyde hydrolase, 6-isopropyl-HODA hydrolase, CumD, HcdC, HMSH, HOD, HOD hydrolase, hydrolase, 2-hydroxymuconate semialdehyde, hydroxymuconic semialdehyde hydrolase, meta-cleavage compound hydrolase, meta-cleavage product hydrolase, MfhA, TodF, XylF

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.9 2-hydroxymuconate-6-semialdehyde hydrolase

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Results	in table
2	Activating Compound
391	AA Sequence
4	Application
1	CAS Registry Number
8	Cloned(Commentary)
2	Crystallization (Commentary)
6	General Information
19	Inhibitors
3	kcat/KM [mM/s]
74	KM Value [mM]
1	Metals/Ions
14	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
70	Organism
10	Pathway
11	PDB ID
6	pH Optimum
1	pH Range
3	pH Stability
1	pI Value
41	Protein Variants
8	Purification (Commentary)
1	Reaction
1	Reaction Type
33	Reference
10	Specific Activity [micromol/min/mg]
1	Storage Stability
96	Substrates and Products (Substrate)
20	Subunits
51	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
48	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.7.1.9 - 2-hydroxymuconate-6-semialdehyde hydrolase

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1.18

Pseudomonas sp.

G3KFX4

purified recombinant enzyme, substrate 2-hydroxy-5-methylmuconate-6-semialdehyde, pH 7.5, 25°C

10.1

isoenzyme HODHII

12.1

mutant S101A

17.4

isoenzyme HODHI

17.6

mutant D233A

28.1

His-tagged HOD

28.4

purified recombinant enzyme, substrate 2-hydroxy-6-oxohepta-2,4-dienoate, pH 7.5, 25°C

733435

3.87

Pseudomonas sp.

G3KFX4

purified recombinant enzyme, substrate 2-hydroxymuconate-6-semialdehyde, pH 7.5, 25°C

733435

Pseudomonas putida

247052

6.1

Pseudomonas fluorescens

S103A mutant

674958