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3.7.1.9: 2-hydroxymuconate-6-semialdehyde hydrolase

This is an abbreviated version!
For detailed information about 2-hydroxymuconate-6-semialdehyde hydrolase, go to the full flat file.

Word Map on EC 3.7.1.9

Reaction

2-hydroxymuconate-6-semialdehyde
+
H2O
=
formate
+
2-oxopent-4-enoate

Synonyms

1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase, 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase, 2-hydroxymuconate semialdehyde hydrolase, 2-hydroxymuconate-semialdehyde hydrolase, 2-hydroxymuconic semialdehyde hydrolase, 6-isopropyl-HODA hydrolase, CumD, HcdC, HMSH, HOD, HOD hydrolase, hydrolase, 2-hydroxymuconate semialdehyde, hydroxymuconic semialdehyde hydrolase, meta-cleavage compound hydrolase, meta-cleavage product hydrolase, MfhA, TodF, XylF

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.9 2-hydroxymuconate-6-semialdehyde hydrolase

Cloned

Cloned on EC 3.7.1.9 - 2-hydroxymuconate-6-semialdehyde hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expression in Escherichia coli
-
expression in Escherichia coli XL1-Blue M15
-
gene pheD encoded in the phe lower operon of the TOU catabolic pathway, DNA and amino acid sequence determination and analysis, genetic organization, recombinant expression in Escherichia coli strain JM109
into pUC119 and overexpression in Escherichia coli
-
overexpression as an N-terminal His-tag from pSM12 in Escherichia coli BL21(DE3), or from plasmid pSM13 in Pseudomonas aeruginosa PAO1161
-
subcloned in Escherichia coli IPTG inducible vectors
-