3.7.1.8: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

This is an abbreviated version!
For detailed information about 2,6-dioxo-6-phenylhexa-3-enoate hydrolase, go to the full flat file.

Word Map on EC 3.7.1.8

3.7.1.8

biphenyls

hydrolases

dioxygenase

hopdas

benzoate

1,2-dioxygenase

2-hydroxypenta-2,4-dienoate

molecular biology

cumene

oxyanion

2,3-dihydroxybiphenyl

polychlorinated

rhodococcus

sphingomonas

ring-hydroxylating

2-hydroxy-6-oxohepta-2,4-dienoate

2-hydroxymuconic

4-chlorobiphenyl

wittichii

xenovorans

extradiol

ser-his-asp

isopropylbenzene

dibenzofuran

acyl-enzyme

carbanion

degradation

environmental protection

Reaction

Synonyms

2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dieonic acid hydrolase, 6-phenyl HODA hydrolase, BphD, BphD enzyme, BphDP6, CarC, HOHPDA hydrolase, HOPD hydrolase, HOPDA hydrolase, HPDA hydrolase, HsaD, hydrolase, 2,6-dioxo-6-phenylhexa-3-enoate, LigY, MCP hydrolase, meta-cleavage product hydrolase, MhpC

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.8 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

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Results	in table
648	AA Sequence
9	Application
1	CAS Registry Number
16	Cloned(Commentary)
1	Cofactor
8	Crystallization (Commentary)
21	General Information
19	Inhibitors
25	kcat/KM [mM/s]
3	Ki Value [mM]
70	KM Value [mM]
1	Metals/Ions
11	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
83	Organism
7	Pathway
40	PDB ID
6	pH Optimum
3	pH Range
2	pH Stability
53	Protein Variants
15	Purification (Commentary)
8	Reaction
1	Reaction Type
44	Reference
6	Specific Activity [micromol/min/mg]
4	Storage Stability
100	Substrates and Products (Substrate)
14	Subunits
76	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
67	Turnover Number [1/s]

Purification

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Purification on EC 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

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4 entries

2 isofunctional hydrolases H1 and H2

Sphingomonas sp.

247042

BphD mutants purified by affinity chromatography to more than 95% homogeneity

Paraburkholderia xenovorans

672081

by metal affinity chromatography

Pseudomonas sp.

676944

by metal affinity chromatography, to more than 90% homogeneity

Paraburkholderia xenovorans

672080

gel filtration, to homogeneity

Bacillus sp. (in: Bacteria)

675835

MhpC mutants purified by anion exchange chromatography to more than 95% homogeneity

Escherichia coli

672081

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

standard method

to apparent homogeneity

Burkholderia cepacia

674405

to more than 90% homogeneitiy

Pseudomonas sp.

676272

to more than 99% homogeneity

Rhodococcus globerulus

674232