3.7.1.8: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
This is an abbreviated version!
For detailed information about 2,6-dioxo-6-phenylhexa-3-enoate hydrolase, go to the full flat file.
Word Map on EC 3.7.1.8
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3.7.1.8
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biphenyls
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hydrolases
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dioxygenase
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hopdas
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benzoate
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1,2-dioxygenase
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2-hydroxypenta-2,4-dienoate
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molecular biology
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cumene
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oxyanion
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2,3-dihydroxybiphenyl
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polychlorinated
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rhodococcus
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sphingomonas
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ring-hydroxylating
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2-hydroxy-6-oxohepta-2,4-dienoate
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2-hydroxymuconic
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4-chlorobiphenyl
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wittichii
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xenovorans
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extradiol
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ser-his-asp
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isopropylbenzene
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dibenzofuran
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acyl-enzyme
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carbanion
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degradation
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environmental protection
- 3.7.1.8
- biphenyls
- hydrolases
- dioxygenase
- hopdas
- benzoate
-
1,2-dioxygenase
- 2-hydroxypenta-2,4-dienoate
- molecular biology
- cumene
-
oxyanion
- 2,3-dihydroxybiphenyl
-
polychlorinated
- rhodococcus
- sphingomonas
-
ring-hydroxylating
- 2-hydroxy-6-oxohepta-2,4-dienoate
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2-hydroxymuconic
- 4-chlorobiphenyl
-
wittichii
- xenovorans
-
extradiol
-
ser-his-asp
-
isopropylbenzene
- dibenzofuran
- acyl-enzyme
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carbanion
- degradation
- environmental protection
Reaction
Synonyms
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dieonic acid hydrolase, 6-phenyl HODA hydrolase, BphD, BphD enzyme, BphDP6, CarC, HOHPDA hydrolase, HOPD hydrolase, HOPDA hydrolase, HPDA hydrolase, HsaD, hydrolase, 2,6-dioxo-6-phenylhexa-3-enoate, LigY, MCP hydrolase, meta-cleavage product hydrolase, MhpC
ECTree
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Crystallization
Crystallization on EC 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
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mutant S114A in complex with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, 8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid, or 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid, sitting drop vapor diffusion, 0.001 ml of 8 mg/ml protein solution is mixed with 0.001 ml of precipitant solution containing 200 mM KSCN, 24% PEG 3350, and 100 mM bis-tris propane, pH 7.0, soaking of S114A crystals in 0.01 ml of precipitant solution supplemented with 15 mM of each ligand for 30-60 min, X-ray diffractin structure determination and analysis at 1.8-2.1 A resolution, molecular replacement
BphD H265Q and S112A/H265Q mutants in complex with substrate 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, sitting drop vapour diffusion method, 0.001 ml of protein solution containing 4 mg/ml protein in 20 mM HEPES, pH 7.5, 20°C, 9 days, is mixed with 0.001 ml of reservoir solution containing 2.4 M malonate at pH 6.0-7.0, X-ray diffraction structure determination and analysis at 1.3 A and 1.9 A resolutions, respectively
enzyme structure determination and analysis, PDB IDs 2OG1, 2PU5, 2RI6, 2PU7, 2PUH and 2PUJ
S112A and H265Q mutants crystal structure analysis, PDB IDs 2PUH and 3V1N, modeling
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wild-type, the S112C variant and S112C incubated with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid by hanging drop vapor diffusion method at 20°C, to 1.6 A resolution, interaction between conserved active side residues and dienoate moiety of the substrate, the residue His265 is hydrogen-bonded to the 2-hydroxy/oxo substituent of the substrate, consistent with a role in catalyzing ketonization
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at 2.4 A resolution, active-site residues are Ser110, Asp235 and His263, situated inside the cavity between the core and the lid domains, this substrate binding pocket has a hydrophobic and hydrophilic region