3.7.1.7: beta-diketone hydrolase
This is an abbreviated version!
For detailed information about beta-diketone hydrolase, go to the full flat file.
Word Map on EC 3.7.1.7
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3.7.1.7
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sphingopyxis
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textile
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putida
-
lipase
-
sludge
-
shake
-
cytochemically
-
ache
-
desizing
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acetylacetone
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gly-x-ser-x-gly
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p-nitrophenyl
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alpha-granules
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caprylate
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industry
-
wastewater
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hydrolysed
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pmsf
-
sphingomonas
-
depolymerases
-
dt
-
molecular biology
- 3.7.1.7
- sphingopyxis
-
textile
- putida
- lipase
-
sludge
-
shake
-
cytochemically
-
ache
-
desizing
- acetylacetone
-
gly-x-ser-x-gly
- p-nitrophenyl
-
alpha-granules
- caprylate
- industry
-
wastewater
-
hydrolysed
- pmsf
- sphingomonas
-
depolymerases
- dt
- molecular biology
Reaction
Synonyms
ABDH, Alr4455 protein, beta-diketone hydrolase, OPH, OPH hydrolase, oxidized polyvinyl alcohol hydrolase, oxidized PVA hydrolase, oxidized-PVA hydrolase, poly(vinyl alcohol) (PVA)-degrading enzyme, pOPH, PVA-degrading enzyme, PVAase, sOPH
ECTree
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Purification
Purification on EC 3.7.1.7 - beta-diketone hydrolase
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recombinant enzyme 1-67fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, and hydrophobic interaction and cation exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 trxB (DE3) by nickel affinity chromatography and digestion by TEV protease to remove the His-tagged thioredoxin, followed by a second nickel affinity chromatography, and anion exchange chromatography and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
using a nickel affinity HiTRAP agarose column and a S200 16/60 Sephadex column