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3.7.1.7: beta-diketone hydrolase

This is an abbreviated version!
For detailed information about beta-diketone hydrolase, go to the full flat file.

Word Map on EC 3.7.1.7

Reaction

Nonane-4,6-dione
+
H2O
=
Pentan-2-one
+
Butanoate

Synonyms

ABDH, Alr4455 protein, beta-diketone hydrolase, OPH, OPH hydrolase, oxidized polyvinyl alcohol hydrolase, oxidized PVA hydrolase, oxidized-PVA hydrolase, poly(vinyl alcohol) (PVA)-degrading enzyme, pOPH, PVA-degrading enzyme, PVAase, sOPH

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.7 beta-diketone hydrolase

Purification

Purification on EC 3.7.1.7 - beta-diketone hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme 1-67fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, and hydrophobic interaction and cation exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 trxB (DE3) by nickel affinity chromatography and digestion by TEV protease to remove the His-tagged thioredoxin, followed by a second nickel affinity chromatography, and anion exchange chromatography and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
to homogeneity, 5-9fold, gel filtration
-
using a nickel affinity HiTRAP agarose column and a S200 16/60 Sephadex column