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3.7.1.7: beta-diketone hydrolase

This is an abbreviated version!
For detailed information about beta-diketone hydrolase, go to the full flat file.

Word Map on EC 3.7.1.7

Reaction

Nonane-4,6-dione
+
H2O
=
Pentan-2-one
+
Butanoate

Synonyms

ABDH, Alr4455 protein, beta-diketone hydrolase, OPH, OPH hydrolase, oxidized polyvinyl alcohol hydrolase, oxidized PVA hydrolase, oxidized-PVA hydrolase, poly(vinyl alcohol) (PVA)-degrading enzyme, pOPH, PVA-degrading enzyme, PVAase, sOPH

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.7 beta-diketone hydrolase

Crystallization

Crystallization on EC 3.7.1.7 - beta-diketone hydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the structure of beta-diketone hydrolase is determined to a resolution of 1.5 A in both native and ligand-bound forms
purified recombinant detagged wild-type enzyme and mutants S172A and S172C, free and in complexes with PEG, butyrate, or DMSO, respectively, by sitting drop method using 30% PEG 5000 MME, 0.1 M MES pH 6.5. and 0.2 M (NH4)2SO4 for the wild-type, and 0.1 M tri-sodium citrate, pH 5.6, 30% w/v PEG 4000, and 0.3% w/v n-octyl-beta-D-glucoside for the mutants, X-ray diffraction structure determination and analysis at 1.49-2.10 A resolution, molecular replacement