3.7.1.7: beta-diketone hydrolase
This is an abbreviated version!
For detailed information about beta-diketone hydrolase, go to the full flat file.
Word Map on EC 3.7.1.7
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3.7.1.7
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sphingopyxis
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textile
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putida
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lipase
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sludge
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shake
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cytochemically
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ache
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desizing
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acetylacetone
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gly-x-ser-x-gly
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p-nitrophenyl
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alpha-granules
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caprylate
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industry
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wastewater
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hydrolysed
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pmsf
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sphingomonas
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depolymerases
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dt
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molecular biology
- 3.7.1.7
- sphingopyxis
-
textile
- putida
- lipase
-
sludge
-
shake
-
cytochemically
-
ache
-
desizing
- acetylacetone
-
gly-x-ser-x-gly
- p-nitrophenyl
-
alpha-granules
- caprylate
- industry
-
wastewater
-
hydrolysed
- pmsf
- sphingomonas
-
depolymerases
- dt
- molecular biology
Reaction
Synonyms
ABDH, Alr4455 protein, beta-diketone hydrolase, OPH, OPH hydrolase, oxidized polyvinyl alcohol hydrolase, oxidized PVA hydrolase, oxidized-PVA hydrolase, poly(vinyl alcohol) (PVA)-degrading enzyme, pOPH, PVA-degrading enzyme, PVAase, sOPH
ECTree
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Cloned
Cloned on EC 3.7.1.7 - beta-diketone hydrolase
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expression of the OPH gene lacking the sequence encoding the original signal peptide in Escherichia coli strain BL21 (DE3), the recombinant enzyme is secreted, productivity of recombinant OPH reaches 565.95 U/L/h without and 733.17 U/L/h in presence of 200 mM glycine. Subcloning in Escherichia coli strain JM109. Therecombinant extracellular enzyme inhibits growth of Escherichia coli cells
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gene oph, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene oph, recombinant methano-inducible expression of the enzyme, ligated into the pPIC9K vector behind the alpha-factor signal sequence, in Pichia pastoris strain GS115
into the pETYSBLIC vector for expression in Escherichia coli BL21DE3 cells
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 trxB (DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)