3.7.1.4: phloretin hydrolase

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For detailed information about phloretin hydrolase, go to the full flat file.

Word Map on EC 3.7.1.4

3.7.1.4

eubacterium

ramulus

brush

flavonoid

border

medicine

2,4-diacetylphloroglucinol

lactase-phlorizin

molecular biology

lactase

pyridoxine

phloridzin

flavone

beta-glucosidic

carbon-carbon

beta-glucosidase

fluorescens

deglycosylated

mucosal

5'-phosphate

fecal

dihydrochalcone

pyridoxal

abscessus

neohesperidin

Reaction

Synonyms

C-acylphenol acylhydrolase, lactase phlorizin hydrolase, lactase phlorizin-hydrolase, lactase-phlorizin hydrolase, lactase/phlorizin hydrolase, LCT, LPH, Phlg, phloretin hydrolase, Phy, PNG hydrolase

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.4 phloretin hydrolase

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Results	in table
11	Activating Compound
22	AA Sequence
7	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Crystallization (Commentary)
10	General Information
34	Inhibitors
5	kcat/KM [mM/s]
4	Ki Value [mM]
17	KM Value [mM]
14	Localization
5	Metals/Ions
8	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
31	Organism
6	pH Optimum
1	pH Range
3	Posttranslational Modification
29	Protein Variants
11	Purification (Commentary)
5	Reaction
9	Reaction Type
28	Reference
17	Source Tissue
8	Specific Activity [micromol/min/mg]
80	Substrates and Products (Substrate)
9	Subunits
50	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Stability [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 3.7.1.4 - phloretin hydrolase

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5 entries

300fold from intestine brushh border membrane

Rattus norvegicus

656061

Ni-NTA column chromatography and Superdex S200 gel filtration

Mycobacteroides abscessus

750528

recombinant enzyme 13.2fold from Escherichia coli strain DH5alpha(pPH3) by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration

Eubacterium ramulus

Q715L4

654284

recombinant enzyme from Escherichia coli, about 13.2fold

Eubacterium ramulus

Q715L4

654284

recombinant mature enzyme and pro-enzyme from COS-1 cells

Homo sapiens

656110

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Eubacterium ramulus

Q715L4

735304