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3.7.1.4: phloretin hydrolase

This is an abbreviated version!
For detailed information about phloretin hydrolase, go to the full flat file.

Word Map on EC 3.7.1.4

Reaction

phloretin
+
H2O
=
phloretate
 +
phloroglucinol

Synonyms

C-acylphenol acylhydrolase, lactase phlorizin hydrolase, lactase phlorizin-hydrolase, lactase-phlorizin hydrolase, lactase/phlorizin hydrolase, LCT, LPH, Phlg, phloretin hydrolase, Phy, PNG hydrolase

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.4 phloretin hydrolase

Inhibitors

Inhibitors on EC 3.7.1.4 - phloretin hydrolase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-bromopyrrolidine-2,5-dione
3% inhibition at 1 mM
2',4'-dinitrophenyl-3-fluoro-2-deoxy-beta-D-glucopyranoside
-
specific 100fold inhibition of phloretin and lactose hydrolase activity, no inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
2,4-diacetylphloroglucinol
-
competitive inhibition
3-(4-hydroxyphenyl)propionic acid
i.e. phloretate, 30% product inhibition at 0.12 mM; product inhibition
Ag+
-
0.02 mM
Al2(SO4)3
-
1 mM
beta-D-glucose
-
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
brain cerebroside
-
competitive inhibition
-
cellobiose
-
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity
CuCl2
85% inhibition at 1 mM
gluconolactone
-
L-cysteine
-
in presence of p-chloromercuribenzoate, 0.05 mM
lactose
-
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, competitive
maltose
-
inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, inhibition is reduced to 60-75% by addition of 1-deoxynojirimycin
N-bromosuccinimide
97% inhibition at 1 mM
N-ethylmaleimide
65% inhibition at 1 mM
Na2MoO4
-
1 mM
Na2WO4
-
1 mM
NEM
35% inhibition at 1 mM
o-phenanthroline
20% inhibition at 1 mM
p-chloromercuribenzoate
-
0.05 mM
phlorizin
-
competitive inhibition
phloroglucinol
46% product inhibition at 0.12 mM; product inhibition
thyroid hormone
-
LPH mRNA level and lactase activity repressed by the injection of thyroid hormones during starvation
-
Zn2+
-
0.2 mM
additional information
-
no inhibition by pyridoxine, trehalose, and sucrose
-