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3.7.1.4: phloretin hydrolase

This is an abbreviated version!
For detailed information about phloretin hydrolase, go to the full flat file.

Word Map on EC 3.7.1.4

Reaction

phloretin
+
H2O
=
phloretate
+
phloroglucinol

Synonyms

C-acylphenol acylhydrolase, lactase phlorizin hydrolase, lactase phlorizin-hydrolase, lactase-phlorizin hydrolase, lactase/phlorizin hydrolase, LCT, LPH, Phlg, phloretin hydrolase, Phy, PNG hydrolase

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.4 phloretin hydrolase

Cloned

Cloned on EC 3.7.1.4 - phloretin hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha
expressed in COS-1 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
gene phy, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene phy, encoded in the 2,4-diacetylphloroglucinol operon, library screening, DNA and amino acid sequence determination and analysis, sequence comparisons and promoter sequence determination, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha(pPH3)
generation of a galectin-3 knock-out mutant
-
the C/T-13910 SNP in the gene encoding lactase-phlorizin hydrolase on chromosome 2 defines adult-type hypolactasia status. Study in young finns analysing association of adult-type hypolactasia with coronary artery disease risk factors and with with markers of early atherosclerosis respectively
-
transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation
-
transient transfection of deletion constructs in both LPH-expressing Caco-2 and LPH-nonexpressing Hep-G2 cells, and in HeLa cells
-