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3.7.1.2: fumarylacetoacetase

This is an abbreviated version!
For detailed information about fumarylacetoacetase, go to the full flat file.

Word Map on EC 3.7.1.2

Reaction

4-Fumarylacetoacetate
+
H2O
=
acetoacetate
 +
fumarate

Synonyms

4-fumarylacetoacetate fumarylhydrolase, acylpyruvase, AtFAH, beta-diketonase, diketo acid hydrolase, EaFAH, FAA, FAA hydrolase, FAH, fumarylacetoacetase, fumarylacetoacetate hydrolase, HmgB, mFAH, NagK, SSCD1, TTHA0809

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.2 fumarylacetoacetase

Cloned

Cloned on EC 3.7.1.2 - fumarylacetoacetase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of FAH genomic DNA and cDNA by degenerate PCR and RACE method, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) from pET28a-FAH
expressed in Escherichia coli
-
expression as glutathione S-transferase fusion protein
-
expression in BMT-10 cells
-
expression in CV-1 transfected cells
-
expression of C-terminally His-tagged enzyme in a bacterial expression system
-
for expression in Escherichia coli
generation of a Fah-knockout mutant as a model of hereditary tyrosinemia type I. Transplantation of wild-type bone marrow cells from fully allogeneic BALB/c donors into Fah-knockout mutant after lethal total body irradiation results in healthy recipients without pharmacological support
into the vector pET11a for expression in Escherichia coli BL21DE3 cells
-
proteome analysis. FAH protein level is down-regulated in the Fe2+ adequate fed TNF-deltaARE mutant and up-regulated in the Fe2+ low fed TNF-deltaARE mutant compared to wild-type
-
SSCD1 gene, DNA and amino acid sequence determination and analysis, map-based cloning