3.7.1.18: 6-oxocamphor hydrolase
This is an abbreviated version!
For detailed information about 6-oxocamphor hydrolase, go to the full flat file.
Word Map on EC 3.7.1.18
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3.7.1.18
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crotonase
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desymmetrization
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beta-diketones
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bicyclic
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diketone
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rhodococcus
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ncimb
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prochiral
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enol
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hexamer
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anabaena
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trimer
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dyad
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carbon-carbon
- 3.7.1.18
- crotonase
-
desymmetrization
- beta-diketones
-
bicyclic
- diketone
- rhodococcus
-
ncimb
-
prochiral
-
enol
-
hexamer
- anabaena
- trimer
-
dyad
-
carbon-carbon
Reaction
Synonyms
6-oxo camphor hydrolase, CaMK, OCH, retro-Claisenase
ECTree
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Crystallization
Crystallization on EC 3.7.1.18 - 6-oxocamphor hydrolase
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selenomethionine-labeled enzyme, vapor diffusion hanging drop technique, protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.0, 1 mM dithiothreitol, 0.020 mM phenylmethylsulfonyl fluoride is mixed in a 1:1 ratio with reservoir solution, that contains for the native enzyme, the reservoir solution consisted of 0.1 M sodium acetate buffer, pH 4.5, 0.2 M ammonium sulfate, and 28% v/v PEG 4000, and for the selenomethionyl enzyme 0.1 M MES, pH 5.5, 0.2 M ammonium sulfate, 37.5% v/v 5,000 Da monomethyl ether, and 0.2% w/v n-octyl-beta-D-glucopyranoside, X-ray diffraction structure determination and analysis at 2.0-2.4 A resolution, selenomethionine multiple wave anomalous dispersion, molecular modeling
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OCH mutant H122A in complex with the minor diastereoisomer of (2S,4S)-alpha-campholinic acid, vapor diffusion hanging drop technique, mxing of protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.1, 1 mM dithiothreitol, and 0.02 mM phenylmethylsulfonyl fluoride with reservoir solution containing 0.1 M 2-(N-morpholino)ethanesulfonic acid, pH 5.6, 0.2 M calcium acetate, and 26% v/v PEG monomethyl ether 2000, in a 1:1 ratio, soakong of crystals in reservoir solution containing 6-oxocamphor for 30 min, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement