3.7.1.17: 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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For detailed information about 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase, go to the full flat file.

Reaction

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate

3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate

Synonyms

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, HsaD, TesD

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.17 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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Results	in table
476	AA Sequence
5	Cloned(Commentary)
2	Crystallization (Commentary)
1	IC50 Value
14	Inhibitors
4	kcat/KM [mM/s]
5	KM Value [mM]
2	Localization
2	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
582	Organism
4	Pathway
14	PDB ID
1	pH Optimum
1	Protein Variants
4	Purification (Commentary)
1	Reaction
10	Reference
13	Substrates and Products (Substrate)
4	Subunits
13	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.7.1.17 - 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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Go to Crystallization (commentary) Search

mutant enzyme S114A, sitting drop vapor diffusion method, using 200 mM KSCN, 24% polyethylene glycol 3.35 K, and 100 mM bis-tris propane, pH 7.0

Mycobacterium tuberculosis

717815

sitting drop vapor diffusion method, using 30% (w/v) PEG 3000, 0.1?M CHES, pH 9.5

Mycobacterium tuberculosis

717030