3.7.1.14: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase

This is an abbreviated version!
For detailed information about 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, go to the full flat file.

Reaction

Synonyms

2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase, MCP hydrolase, meta-cleavage product hydrolase, MhpC

ECTree

     3 Hydrolases

         3.7 Acting on carbon-carbon bonds

             3.7.1 In ketonic substances

                3.7.1.14 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase

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Results	in table
1222	AA Sequence
4	Cloned(Commentary)
4	Crystallization (Commentary)
1	Expression
7	General Information
1	Inhibitors
16	kcat/KM [mM/s]
16	KM Value [mM]
3	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
9	Organism
7	Pathway
3	pH Optimum
14	Protein Variants
2	Purification (Commentary)
5	Reaction
9	Reference
18	Substrates and Products (Substrate)
2	Subunits
14	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
17	Turnover Number [1/s]

Engineering

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Engineering on EC 3.7.1.14 - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C261A

Escherichia coli

P77044

1.4fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

F173D

Escherichia coli

P77044

158fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

F173G

Escherichia coli

P77044

32fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

H114A

2 entries

H263A

Escherichia coli

P77044

mutant exhibits very slow ketonisation and C-C cleavage

715899

N109A

Escherichia coli

P77044

125fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

N109H

Escherichia coli

P77044

217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

R188K

Escherichia coli

P77044

217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

R188Q

Escherichia coli

P77044

2941fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

S110A

Escherichia coli

P77044

exhibits fast ketonisation, an intermediate phase, and slow C-C cleavage

715899

S110G

Escherichia coli

P77044

exhibits fast ketonisation, an intermediate phase, and slow C-C cleavage

715899

S40A

Escherichia coli

P77044

shows twofold reduced catalytic efficiency, but shows a very fast interconversion of dienol to dienolate forms of the substrate

715899

W264G

Escherichia coli

P77044

196fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate

672081

H114A

Escherichia coli

-

MhpC mutant is able to accept the 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid, on a shorter time scale

672080

H114A

Escherichia coli

P77044

shows twofold-reduced catalytic efficiency, ruling out a catalytic role, but shows a fivefold-reduced KM for the natural substrate, and an ability to process an aryl-containing substrate, implying a role for His114 in positioning of the substrate

715899