3.7.1.14: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
This is an abbreviated version!
For detailed information about 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, go to the full flat file.
Reaction
Synonyms
2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase, 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase, MCP hydrolase, meta-cleavage product hydrolase, MhpC
ECTree
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Crystallization
Crystallization on EC 3.7.1.14 - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
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hanging drop vapor diffusion method, the X-ray structure of a succinate-H263A MhpC complex shows concerted movements in the positions of both Phe173 and Trp264 that line the approach to Arg188
hanging-drop vapour-diffusion method. The 2.1 A resolution X-ray structure of the native enzyme determined from orthorhombic crystals confirms that it is a member of the alpha/beta hydrolase fold family, comprising eight beta-strands interconnected by loops and helices. The 2.8 A resolution structure of the enzyme cocrystallized with the non-hydrolysable substrate analogue 2,6-diketo-nona-1,9-dioic acid confirms the location of the active site in a buried channel including Ser110, His263 and Asp235, postulated contributors to a serine protease-like catalytic triad in homologous enzymes