3.7.1.11: cyclohexane-1,2-dione hydrolase

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Reaction

Synonyms

Cdh, ThDP-dependent cyclohexane-1,2-dione hydrolase, thiamine diphosphate dependent cyclohexane-1,2-dione hydrolase

ECTree

     3 Hydrolases

         3.7 Acting on carbon-carbon bonds

             3.7.1 In ketonic substances

                3.7.1.11 cyclohexane-1,2-dione hydrolase

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Results	in table
1	Activating Compound
12	AA Sequence
4	Application
2	Cloned(Commentary)
13	Cofactor
2	Crystallization (Commentary)
16	General Information
4	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
4	Metals/Ions
6	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
48	Organism
2	Pathway
4	PDB ID
5	pH Optimum
11	Protein Variants
2	Purification (Commentary)
10	Reaction
16	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
36	Substrates and Products (Substrate)
8	Subunits
12	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.7.1.11 - cyclohexane-1,2-dione hydrolase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native CDH 6.6fold by two different steps of anion exchange chromatography, followed by gel filtration

Azoarcus sp.

P0CH62

719735

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Azoarcus sp.

P0CH62

733657