3.7.1.11: cyclohexane-1,2-dione hydrolase

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For detailed information about cyclohexane-1,2-dione hydrolase, go to the full flat file.

Reaction

Synonyms

Cdh, ThDP-dependent cyclohexane-1,2-dione hydrolase, thiamine diphosphate dependent cyclohexane-1,2-dione hydrolase

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.11 cyclohexane-1,2-dione hydrolase

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Results	in table
1	Activating Compound
12	AA Sequence
4	Application
2	Cloned(Commentary)
13	Cofactor
2	Crystallization (Commentary)
16	General Information
4	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
4	Metals/Ions
6	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
48	Organism
2	Pathway
4	PDB ID
5	pH Optimum
11	Protein Variants
2	Purification (Commentary)
10	Reaction
16	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
36	Substrates and Products (Substrate)
8	Subunits
12	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.7.1.11 - cyclohexane-1,2-dione hydrolase

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crystal structure analysis of CDH in complex with substrate cyclohexane-1,2-dione, PDB ID 2PGN

Azoarcus sp.

P0CH62

735301

purified CDH from strain 22Lin, hanging drop vapour diffusion method, equal volumes of protein storage and reservoir solution, the latter composed of 60% MPD, 20 mM Na+ acetate and 200 mM NaCl, 25°C, a second cubic crystal form is obtained at 20°C with a reservoir solution containing 17-18% w/v PEG 8000, 100 mM Na+ acetate, 10 mM MnCl2, and 2% w/v isopropanol in 0.1 M HEPES buffer, pH 7.5, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling

Azoarcus sp.

P0CH62

719464