3.7.1.1: oxaloacetase

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For detailed information about oxaloacetase, go to the full flat file.

Reaction

Synonyms

(2R,3S)-dimethylmalate lyase, An07g08390, BbOAH, DMML, FpOAH, OAH, OAH-active petal death protein, OAH-like protein, OXA, oxalacetase, oxalacetate acetylhydrolase, oxalacetic hydrolase, oxaloacetase, oxaloacetate acethylhydrolase, oxaloacetate acetylhydrolase, oxaloacetate hydrolase

ECTree

     3 Hydrolases

         3.7 Acting on carbon-carbon bonds

             3.7.1 In ketonic substances

                3.7.1.1 oxaloacetase

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Results	in table
10	AA Sequence
3	Application
1	CAS Registry Number
13	Cloned(Commentary)
2	Crystallization (Commentary)
15	General Information
1	General Stability
8	Inhibitors
6	kcat/KM [mM/s]
4	Ki Value [mM]
37	KM Value [mM]
2	Localization
10	Metals/Ions
11	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
34	Organism
3	Pathway
9	pH Optimum
3	pH Range
1	pH Stability
10	Protein Variants
6	Purification (Commentary)
2	Reaction
7	Reaction Type
31	Reference
9	Source Tissue
10	Specific Activity [micromol/min/mg]
2	Storage Stability
29	Substrates and Products (Substrate)
7	Subunits
45	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
37	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.7.1.1 - oxaloacetase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

DMML in complex with Mg2+ and in complex with Mg2+ and 3,3-difluorooxalacetate, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane (pH 7.5), and 20k polyethylene glycol 3350, or using 14k polyethylene glycol 6000 and 0.1 M MES (pH 6.5)

Aspergillus niger

-

693684

OAH alone, in complex with a inhibitor 3,3-difluorooxalacetate, and in complex with reaction product oxalate, soaking of ligand-free OAH crystals in solution containing 0.2 M MnCl2 or 0.2 M MgCl2, 10 mM CaCl2, 2 mM 3,3-difluorooxalacetate, 0.1 M Na-HEPES, pH 7.5, and 30% v/v PEG 400 for 5-10 min yielded the desired enzyme inhibitor complex, X-ray diffraction structure determination and analysis at resolution limit of 1.30, 1.55, and 1.65 A , respectively, molecular replacement

Cryphonectria parasitica

D5LIR7

719879